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潜伏性结核感染患者循环自然杀伤细胞免疫状态的变化

Changes in immune status of circulating NK cells in patients with latent tuberculosis infection.

作者信息

Qin Shuang, Chen Ruiqi, Li Meihui, Lv Jiangfeng, Zhang Fengmei, Ren Yuguo, Lin Xiangyang

机构信息

Department of Clinical Laboratory, Jinan People's Hospital Affiliated to Shandong First Medical University, China.

Department of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, China.

出版信息

Cent Eur J Immunol. 2024;49(2):105-112. doi: 10.5114/ceji.2024.142104. Epub 2024 Aug 12.

DOI:10.5114/ceji.2024.142104
PMID:39381552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11457571/
Abstract

INTRODUCTION

The presence of patients with latent tuberculosis infection (LTBI) has fueled the tuberculosis pandemic. We aimed to investigate the immune status of NK cells in LTBI patients.

MATERIAL AND METHODS

Twenty-one LTBI patients, 25 active pulmonary tuberculosis (APTB) patients and 25 healthy controls (HCs) participated in our research. The markers of NK cells were detected by flow cytometry.

RESULTS

The absolute number of circulating CD56bright and CD56dim NK cells in LTBI patients was higher than that of APTB patients, but the frequency of HLA-DR+ CD56bright NK cells was significantly lower than that of HCs and APTB patients. Also, LTBI patients with CD56bright NK cells had intracellular levels of granzyme B that were as significantly elevated as those with APTB patients, but the levels of granzyme A and perforin were reduced. Meanwhile, the frequencies of CXCR3+ NK cells, CXCR3+ CD56bright and CXCR3+ CD56dim NK cells were significantly lower in LTBI patients.

CONCLUSIONS

Circulating CD56bright NK cells exerted a significant role in maintaining immune balance in LTBI patients. An elevated frequency of granzyme B+ CD56bright NK cells and a reduced frequency of perforin+ CD56bright NK cells were effective in differentiating LTBI patients from HCs.

摘要

引言

潜伏性结核感染（LTBI）患者的存在加剧了结核病的流行。我们旨在研究LTBI患者中自然杀伤细胞（NK细胞）的免疫状态。

材料与方法

21例LTBI患者、25例活动性肺结核（APTB）患者和25例健康对照（HCs）参与了我们的研究。通过流式细胞术检测NK细胞标志物。

结果

LTBI患者循环中CD56bright和CD56dim NK细胞的绝对数量高于APTB患者，但HLA-DR + CD56bright NK细胞的频率显著低于HCs和APTB患者。此外，具有CD56bright NK细胞的LTBI患者颗粒酶B的细胞内水平与APTB患者一样显著升高，但颗粒酶A和穿孔素的水平降低。同时，LTBI患者中CXCR3 + NK细胞、CXCR3 + CD56bright和CXCR3 + CD56dim NK细胞的频率显著降低。

结论

循环中的CD56bright NK细胞在维持LTBI患者的免疫平衡中发挥了重要作用。颗粒酶B + CD56bright NK细胞频率升高和穿孔素 + CD56bright NK细胞频率降低有助于区分LTBI患者和HCs。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/89d6c37afe82/CEJI-49-54595-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/f9cb490114b8/CEJI-49-54595-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/86b75e34c968/CEJI-49-54595-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/3c88461e2e78/CEJI-49-54595-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/232f2ff35faf/CEJI-49-54595-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/89d6c37afe82/CEJI-49-54595-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/f9cb490114b8/CEJI-49-54595-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/86b75e34c968/CEJI-49-54595-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/3c88461e2e78/CEJI-49-54595-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/232f2ff35faf/CEJI-49-54595-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb8/11457571/89d6c37afe82/CEJI-49-54595-g005.jpg

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Multifunctional T cell response in active pulmonary tuberculosis patients.
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