Goralogia Greg S, Howe Glenn T, Brunner Amy M, Helliwell Emily, Nagle Michael F, Ma Cathleen, Lu Haiwei, Goddard Amanda L, Magnuson Anna C, Klocko Amy L, Strauss Steven H
Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR, USA.
Department of Forest Resources and Environmental Conservation, Virginia Tech, Blacksburg, VA, USA.
Hortic Res. 2021 Aug 1;8(1):167. doi: 10.1038/s41438-021-00600-4.
The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants, particularly for wind-pollinated trees such as poplar. We report that overexpression of a poplar homolog of the floral repressor SHORT VEGETATIVE PHASE-LIKE (SVL), a homolog of the Arabidopsis MADS-box repressor SHORT VEGETATIVE PHASE (SVP), delayed the onset of flowering several years in three genotypes of field-grown transgenic poplars. Higher expression of SVL correlated with a delay in flowering onset and lower floral abundance, and did not cause morphologically obvious or statistically significant effects on leaf characteristics, tree form, or stem volume. Overexpression effects on reproductive and vegetative phenology in spring was modest and genotype-specific. Our results suggest that use of SVL and related floral repressors can be useful tools to enable a high level of containment for vegetatively propagated short-rotation woody energy or pulp crops.
转基因和外来种质从种植作物扩散到野生或野生近缘物种，是公众和监管部门接受转基因植物时面临的一个难题，对于杨树等风媒授粉的树木而言尤其如此。我们报告称，拟南芥MADS盒抑制因子短营养生长期（SVP）的同源物、花抑制因子短营养生长期样蛋白（SVL）的杨树同源物过表达，使三种基因型的田间种植转基因杨树的开花起始延迟了数年。SVL的较高表达与开花起始延迟和花量减少相关，并且对叶片特征、树形或茎体积没有造成形态学上明显或统计学上显著的影响。过表达对春季生殖和营养物候的影响较小且具有基因型特异性。我们的结果表明，使用SVL和相关花抑制因子可以成为对无性繁殖的短轮伐期木质能源或纸浆作物进行高水平隔离的有用工具。
