Kang Yei-Jin, Kim Seong-Gon
Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Jibyun-dong, Gangneung, Gangwondo, 28644, Republic of Korea.
Maxillofac Plast Reconstr Surg. 2021 Aug 1;43(1):28. doi: 10.1186/s40902-021-00314-6.
In stress situations, bacteria produce dormancy-inducing factors to stop cell growth. The dormancy-inducing factors may have an inhibitory effect on tumor cell growth. Here we analyzed the differentially expressed protein profiles after 4-hexylresorcinol (4HR), one of the dormancy-inducing factors, administration using in vitro oral squamous carcinoma cells (SCC-9).
The control group was SCC-9 cells culture without 4HR administration. The experimental group received 10 μg/mL of 4HR. Collected proteins from each group were loaded for 2D electrophoresis. Among the separated proteins, 20 differentially expressed proteins were selected and processed for LC-MS/MS.
In proteomic analysis, the expression of keratin 1, keratin 10, and histone H2B were increased. In cDNA microarray assay, the genes related to the cellular differentiation (involucrin, keratin 13, 14) were highly expressed in the 4HR treated group (fold ratio > 2.0; Table 2). Interestingly, histone family was upregulated in the cDNA microarray assay.
The administration of 4HR on SCC-9 cells increased epithelial cell differentiation markers and histone.
在应激情况下，细菌会产生诱导休眠的因子来停止细胞生长。这些诱导休眠的因子可能对肿瘤细胞生长具有抑制作用。在此，我们使用体外口腔鳞状癌细胞（SCC-9）分析了诱导休眠因子之一的4-己基间苯二酚（4HR）给药后的差异表达蛋白质谱。
对照组为未给予4HR的SCC-9细胞培养物。实验组接受10μg/mL的4HR。将每组收集的蛋白质进行二维电泳。从分离出的蛋白质中选择20种差异表达蛋白质并进行液相色谱-串联质谱分析（LC-MS/MS）。
在蛋白质组学分析中，角蛋白1、角蛋白10和组蛋白H2B的表达增加。在cDNA微阵列分析中，与细胞分化相关的基因（包壳蛋白、角蛋白13、14）在4HR处理组中高表达（折叠比>2.0；表2）。有趣的是，在cDNA微阵列分析中组蛋白家族上调。
对SCC-9细胞给予4HR可增加上皮细胞分化标志物和组蛋白。
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