4-己基间苯二酚给药对生长大鼠下颌下腺的影响。

Effects of 4-hexylresorcinol administration on the submandibular glands in a growing rat model.

机构信息

Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Jukheon gil 7, 25457, Gangneung, Gangwondo, Republic of Korea.

Department of Oral Biochemistry, College of Dentistry, Gangneung-Wonju National University, 28644, Gangneung, Korea.

出版信息

Head Face Med. 2022 Jun 6;18(1):16. doi: 10.1186/s13005-022-00320-7.

DOI:10.1186/s13005-022-00320-7

PMID:35668488

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9172157/

Abstract

BACKGROUND

4-Hexylresorcinol (4HR) is a food additive and class I histone deacetylase inhibitor. In this study, we examined the effects of 4HR administration on the submandibular gland in a growing rat model.

METHODS

Four-week-old rats were used in this study. The experimental group (nine males and eight females) received 12.8 mg/kg of 4HR weekly for 12 weeks. Ten rats (five males and five females) were used as controls. The submandibular glands of rats were collected 12 weeks after the first administration of 4HR. The weight of the glands was measured. Histological analysis, immunoprecipitation-high-performance liquid chromatography (IP-HPLC), and western blotting were performed.

RESULTS

The weights of the rat submandibular glands were higher in the experimental groups than in the control group, especially in male rats (P < 0.05). The vascular endothelial growth factor (VEGF) and testosterone in the submandibular glands were more highly expressed in 4HR-treated male rats than in untreated rats, as detected by both western blotting and immunohistochemistry. The IP-HPLC results demonstrated that the expression levels of Ki67, epidermal growth factor, and testosterone in the submandibular glands were higher in 4HR-treated male rats than in untreated rats.

CONCLUSIONS

This study demonstrated that the systemic administration of 4HR increased the weight of submandibular glands in male rats. In addition, the testosterone and VEGF expression levels in the submandibular glands increased owing to 4HR administration.

摘要

背景

4-己基间苯二酚（4HR）是一种食品添加剂和 I 类组蛋白去乙酰化酶抑制剂。在这项研究中，我们检查了 4HR 给药对生长大鼠模型下颌下腺的影响。

方法

本研究使用 4 周龄大鼠。实验组（9 只雄性和 8 只雌性）每周接受 12.8mg/kg 的 4HR，共 12 周。10 只大鼠（5 只雄性和 5 只雌性）作为对照组。4HR 首次给药 12 周后收集大鼠的下颌下腺。测量腺体的重量。进行组织学分析、免疫沉淀高效液相色谱（IP-HPLC）和蛋白质印迹分析。

结果

实验组大鼠的下颌下腺重量高于对照组，尤其是雄性大鼠（P<0.05）。Western blot 和免疫组化检测显示，4HR 处理的雄性大鼠下颌下腺中的血管内皮生长因子（VEGF）和睾酮表达水平更高。IP-HPLC 结果表明，4HR 处理的雄性大鼠下颌下腺中的 Ki67、表皮生长因子和睾酮表达水平更高。