Sawczuk I S, Walsh W, King W, Olsson C A, Nguyen-Huu C
Department of Urology, College of Physicians and Surgeons, Columbia University, New York, N.Y.
Urol Int. 1987;42(5):321-5. doi: 10.1159/000281983.
The level of mRNA expression and DNA structure of c-H-ras were examined in a transitional cell carcinoma (TCC) induced in C3H/He mice and Fisher rats by N-(4,5-nitro-2-furylthiazolyl)formamide (FANFT) and carried as serially transplanted tumors. Neither amplification nor gross rearrangements were detected by Southern analysis in the FANFT tumor. Increased expression of c-H-ras was detected by Northern and in situ hybridization in both the FANFT-induced mouse and rat TCC, as compared to normal urothelium. No expression of six other cellular oncogenes was detected in the tumor. It appears that the increase in H-ras expression in the FANFT tumor is specific to this oncogene and that increased expression of the H-ras gene may play a role in bladder carcinogenesis.
在由N-（4,5-硝基-2-呋喃基噻唑基）甲酰胺（FANFT）诱导并作为连续移植瘤传代的C3H/He小鼠和Fisher大鼠的移行细胞癌（TCC）中，检测了c-H-ras的mRNA表达水平和DNA结构。Southern分析在FANFT肿瘤中未检测到扩增或明显重排。与正常尿路上皮相比，通过Northern和原位杂交在FANFT诱导的小鼠和大鼠TCC中均检测到c-H-ras表达增加。在肿瘤中未检测到其他六种细胞癌基因的表达。似乎FANFT肿瘤中H-ras表达的增加是该癌基因特有的，并且H-ras基因表达的增加可能在膀胱癌发生中起作用。
