College of Pharmacy (International Academy of Targeted Therapeutics and Innovation), National & Local Joint Engineering Research Center of Targeted and Innovative Therapeutics, Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, Chongqing University of Arts and Sciences, Chongqing 402160, China.
Dis Markers. 2021 Jul 12;2021:5031479. doi: 10.1155/2021/5031479. eCollection 2021.
Lung adenocarcinoma (LUAD) is the most prevalent histologic type of lung cancer, associated with a high incidence rate and substantial mortality rate worldwide. Accumulating evidence shows that the aberrant expression of neuromedin U (NMU) contributes to the initiation and progression of cancer. Herein, we explored whether NMU could be adopted as a new diagnostic and therapeutic marker in LUAD. The UALCAN and GEPIA web resources were employed to assess data on the NMU expression in LUAD. The STRING web resource was used to develop the PPI (protein-protein interaction) network of NMU, whereas Cytoscape was applied for module analysis. The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of NMU and the interacting proteins were examined using the WebGestalt tool. Survival analysis was performed with the Kaplan-Meier plotter tool. Results revealed that the NMU expression in LUAD was significantly higher than in the nonmalignant tissues. Moreover, higher NMU levels were dramatically related to shorter overall survival, first progression survival, and postprogression survival. The specific gene mutations G45V, R143T, and F152L of NMU occurred in LUAD samples and were associated with a worse prognosis in patients. KEGG and western blot analyses demonstrated an association of NMU with the cell cycle and the cAMP signaling cascade. Bioinformatic analysis and the in vitro experiments implicated NMU as a promising prognostic signature and treatment target for LUAD.
肺腺癌（LUAD）是最常见的肺癌组织学类型，与全球范围内的高发病率和高死亡率相关。越来越多的证据表明，神经调节素 U（NMU）的异常表达有助于癌症的发生和发展。在此，我们探讨了 NMU 是否可以作为 LUAD 的一种新的诊断和治疗标志物。UALCAN 和 GEPIA 网络资源用于评估 LUAD 中 NMU 表达的数据。STRING 网络资源用于开发 NMU 的 PPI（蛋白质-蛋白质相互作用）网络，而 Cytoscape 则用于模块分析。使用 WebGestalt 工具对 NMU 和相互作用蛋白进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。使用 Kaplan-Meier 绘图仪工具进行生存分析。结果表明，LUAD 中的 NMU 表达明显高于非恶性组织。此外，较高的 NMU 水平与总生存期、首次进展生存期和进展后生存期较短显著相关。NMU 的特定基因突变 G45V、R143T 和 F152L 发生在 LUAD 样本中，与患者的预后较差相关。KEGG 和 Western blot 分析表明，NMU 与细胞周期和 cAMP 信号级联有关。生物信息学分析和体外实验表明，NMU 是 LUAD 有前途的预后标志物和治疗靶点。
