Department of Thoracic Surgery, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, 518020 Guangdong, China.
Department of Cardiac Surgery, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, 518020 Guangdong, China.
Biomed Res Int. 2021 Jul 19;2021:6733341. doi: 10.1155/2021/6733341. eCollection 2021.
The study is aimed at investigating the changes in expressions of heat shock protein 27 (HSP27), HSP70, and soluble glycoprotein (SGP) in heart failure (HF) rats complicated with pulmonary edema and exploring their potential correlations with cardiopulmonary functions. The rat model of HF was established, and the rats were divided into HF model group (model group, = 15) and normal group ( = 15). After successful modeling, MRI and ECG were applied to detect the cardiac function indexes of the rats. The myocardial function indexes were determined, the injury of myocardial tissues was observed via hematoxylin and eosin (HE) staining, and the content of myeloperoxidase (MPO), matrix metalloproteinase-9 (MMP-9), and tumor necrosis factor-alpha (TNF-) in the blood was measured. The partial pressure of oxygen (PaO) and oxygenation index (OI) were observed, and the airway resistance and lung compliance were examined. Moreover, quantitative polymerase chain reaction (qPCR) and Western blotting assay were performed to detect the gene and protein expression levels of HSP27, HSP70, and SGP130. The levels of serum creatine kinase (CK), creatine (Cr), and blood urea nitrogen (BUN) were increased markedly in model group ( < 0.05). Model group had notably decreased fractional shortening (FS) and ejection fraction (EF) compared with normal group ( < 0.05), while the opposite results of left ventricular end-diastolic diameter (LVEDD) and left ventricular end-systolic diameter (LVESD) were detected. In model group, the content of serum MPO, MMP-9, and TNF- was raised remarkably ( < 0.05), OI and PaO were reduced notably ( < 0.05), the airway resistance was increased ( < 0.05), and the lung compliance was decreased ( < 0.05). Obviously elevated gene and protein expression levels of HSP27, HSP70, and SGP130 were detected in model group ( < 0.05). The expressions of HSP27, HSP70, and SGP130 are increased in HF rats complicated with pulmonary edema, seriously affecting the cardiopulmonary functions of the rats.
本研究旨在探讨热休克蛋白 27（HSP27）、HSP70 和可溶性糖蛋白（SGP）在心力衰竭（HF）合并肺水肿大鼠中的表达变化，并探讨其与心肺功能的潜在相关性。建立 HF 大鼠模型，将大鼠分为 HF 模型组（模型组，n=15）和正常组（n=15）。造模成功后，应用 MRI 和 ECG 检测大鼠心功能指标，测定心肌功能指标，苏木精-伊红（HE）染色观察心肌组织损伤，检测血液中髓过氧化物酶（MPO）、基质金属蛋白酶-9（MMP-9）和肿瘤坏死因子-α（TNF-α）含量，观察动脉血氧分压（PaO2）和氧合指数（OI），检测气道阻力和肺顺应性。此外，采用实时定量聚合酶链反应（qPCR）和 Western blot 检测 HSP27、HSP70 和 SGP130 的基因和蛋白表达水平。模型组大鼠血清肌酸激酶（CK）、肌酸（Cr）和血尿素氮（BUN）水平明显升高（ < 0.05）。与正常组相比，模型组大鼠的短轴缩短率（FS）和射血分数（EF）明显降低（ < 0.05），而左心室舒张末期直径（LVEDD）和左心室收缩末期直径（LVESD）则相反。模型组大鼠血清 MPO、MMP-9 和 TNF-α含量明显升高（ < 0.05），OI 和 PaO2明显降低（ < 0.05），气道阻力增加（ < 0.05），肺顺应性降低（ < 0.05）。模型组大鼠 HSP27、HSP70 和 SGP130 的基因和蛋白表达水平明显升高（ < 0.05）。HF 合并肺水肿大鼠 HSP27、HSP70 和 SGP130 的表达增加，严重影响大鼠心肺功能。
