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无机多磷酸盐刺激人纤维环细胞中的基质产生。

Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells.

作者信息

Wang Xiangjiang, Gawri Rahul, Lei Changbin, Lee Joon, Sowa Gwendolyn, Kandel Rita, Vo Nam

机构信息

McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh Pennsylvania USA.

Department of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USA.

出版信息

JOR Spine. 2021 Mar 2;4(2):e1143. doi: 10.1002/jsp2.1143. eCollection 2021 Jun.

DOI:10.1002/jsp2.1143
PMID:34337332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8313173/
Abstract

INTRODUCTION

Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, the effects of polyP on human annulus fibrosus (hAF) cell metabolism is not known.

METHODS AND RESULTS

Here, hAF cells cultured in the presence of 0.5 to 1 mM polyP, chain length 22 (polyP-22), showed an increase in glycosaminoglycan content, proteoglycan and collagen synthesis, and aggrecan and collagen type 1 gene expression. Gene expression level of matrix metalloproteinases 1 was reduced while matrix metalloproteinases 3 level was increased in hAF cells treated with 1 mM polyP. Adenosine triphosphate (ATP) synthesis was also significantly increased in hAF cell culture 72 hours after the exposure to 1 mM polyP-22.

CONCLUSIONS

PolyP thus has both anabolic and bioenergetic effects in AF cells, similar to that observed in NP cells. Together, these results suggest polyP as a potential energy source and a metabolic regulator of disc cells.

摘要

引言

无机多聚磷酸盐（polyP）普遍存在于所有生命形式中，是由重复的正磷酸盐单元组成的线性聚合物。此前研究表明，存在于椎间盘组织中的polyP可增加髓核（NP）细胞的细胞外基质生成。然而，polyP对人纤维环（hAF）细胞代谢的影响尚不清楚。

方法与结果

在此，在含有0.5至1 mM链长为22的多聚磷酸盐（polyP-22）的条件下培养的hAF细胞，其糖胺聚糖含量、蛋白聚糖和胶原蛋白合成以及聚集蛋白聚糖和I型胶原蛋白基因表达均有所增加。用1 mM polyP处理的hAF细胞中，基质金属蛋白酶1的基因表达水平降低，而基质金属蛋白酶3的水平升高。在暴露于1 mM polyP-22 72小时后的hAF细胞培养物中，三磷酸腺苷（ATP）合成也显著增加。

结论

因此，polyP在AF细胞中具有合成代谢和生物能量作用，类似于在NP细胞中观察到的情况。这些结果共同表明，polyP是椎间盘细胞的潜在能量来源和代谢调节剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/a2e44ddcfc56/JSP2-4-e1143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/e7416a74e362/JSP2-4-e1143-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/73f26cd5f8f0/JSP2-4-e1143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/9ed180eca344/JSP2-4-e1143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/e2f5e297b23a/JSP2-4-e1143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/4f8bed9af326/JSP2-4-e1143-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/a2e44ddcfc56/JSP2-4-e1143-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/e7416a74e362/JSP2-4-e1143-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/73f26cd5f8f0/JSP2-4-e1143-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/9ed180eca344/JSP2-4-e1143-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/e2f5e297b23a/JSP2-4-e1143-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/4f8bed9af326/JSP2-4-e1143-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918f/8313173/a2e44ddcfc56/JSP2-4-e1143-g003.jpg

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Inorganic polyphosphate is required for sustained free mitochondrial calcium elevation, following calcium uptake.无机多聚磷酸盐是钙摄取后持续升高游离线粒体钙所必需的。
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Oxidative stress-induced senescence markedly increases disc cell bioenergetics.
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