State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
STAR Protoc. 2021 Jul 17;2(3):100496. doi: 10.1016/j.xpro.2021.100496. eCollection 2021 Sep 17.
The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a protocol accessing AMPK activity in mice by measuring glucose uptake induced by AMPK activators, MK8722 and A769662. This protocol can be used to evaluate AMPK signaling under various pathophysiological conditions. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).
腺苷酸活化蛋白激酶（AMPK）是一种主要的营养传感器，也是细胞能量平衡的主要调节剂。一旦被激活，AMPK 会诱导葡萄糖摄取，导致血糖水平短暂下降，可作为 AMPK 活性的指标。在这里，我们通过测量 AMPK 激活剂 MK8722 和 A769662 诱导的葡萄糖摄取来介绍一种检测小鼠 AMPK 活性的方案。该方案可用于评估各种病理生理条件下的 AMPK 信号。有关该方案的使用和执行的完整详细信息，请参见 Jiang 等人。（2021 年）。
