College of Food Science and Engineering, Bohai University, Jinzhou 121013, P. R. China.
Lab of Nutrition and Functional Food, Jilin University, Changchun 130062, P. R. China.
Food Funct. 2021 Sep 20;12(18):8561-8569. doi: 10.1039/d1fo01546j.
The study aimed to investigate potential mechanisms for the anti-hypertensive effects of RVPSL on spontaneously hypertensive rats (SHRs) using a non-targeted metabonomic approach. In this study, UPLC/MS-based non-targeted metabolomics was performed to discover metabolite variation of serum in SHRs with RVPSL treatment. As a result, the serum metabolites of SHRs that were administered RVPSL for four weeks exhibited distinct alterations. Nine potential biomarkers, , choline, adenosine, adrenic acid, L-tryptophan, niacinamide, glycocholic acid, propiolic acid, D-glyceraldehyde 3-phosphate, and phosphoglycolic acid, were significantly altered, which were mainly involved in lipid metabolism, vitamin and amino acid metabolism, purine metabolism, the MAPK signaling pathway, and the renin-angiotensin system. This study suggested that RVPSL potentially exerted potent effects of alleviating hypertension in the SHRs mainly integrated regulations of metabolism and production of choline, L-tryptophan, nicotinamide, and adenosine.
本研究旨在采用非靶向代谢组学方法探究 RVPSL 对自发性高血压大鼠（SHR）的降压作用机制。在本研究中，我们采用 UPLC/MS 非靶向代谢组学方法，发现 RVPSL 处理 SHR 后血清代谢物的变化。结果表明，给予 RVPSL 治疗 4 周的 SHR 血清代谢物发生明显改变。9 个潜在的生物标志物，包括胆碱、腺苷、花生四烯酸、二十二碳五烯酸、L-色氨酸、烟酰胺、甘胆酸、丙炔酸、D-甘油醛 3-磷酸和磷酸甘油酸，均发生显著改变，主要涉及脂类代谢、维生素和氨基酸代谢、嘌呤代谢、MAPK 信号通路和肾素-血管紧张素系统。本研究表明，RVPSL 可能通过调节代谢和胆碱、L-色氨酸、烟酰胺和腺苷的生成，发挥缓解 SHR 高血压的作用。
