Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria,
Clinic for Poultry and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
Avian Dis. 2021 Mar;65(1):165-170. doi: 10.1637/aviandiseases-D-20-00101.
The present report describes outbreaks of subsp. in young geese flocks in Austria. The flocks, comprising 160-1450 goslings of 2-3 wk of age, experienced increased mortalities The clinical signs were characterized by severe central nervous symptoms, namely leg paddling and torticollis. The postmortem investigation revealed hepatitis, splenitis, and a low amount of liquid fluid in the coelomic cavity. Livers were of fragile texture, with white necrotic areas. The latter were also found in spleens. No macroscopic lesions were seen in brains. Bacteriologic investigation followed by bacterial identification by matrix-assisted laser desorption time-of-flight mass spectrometry and phylogenetic analysis of the partial 16S rRNA region revealed the presence in heart, liver, spleen, and brain of subsp. . Histologic investigation revealed multifocal necrosis in liver and spleen samples together with infiltration of mononuclear cells and heterophilic granulocytes. Furthermore, in the lesions, coccoid bacteria could be identified. No histopathologic changes were observed in brain samples from goslings, except in one bird in which accumulation of coccoid bacteria in blood vessels of the brain samples was present. Antibiotic sensitivity tests revealed identical profiles for all strains, which were susceptible to penicillins, cephalosporins, chloramphenicol, imipenem, and tylosin. However, resistance was found against quinolones, aminoglycosides, tetracycline, and trimethoprim-sulfamethoxazole, which are commonly used to treat infections with gram-positive bacteria.
本报告描述了在奥地利年轻鹅群中发生的 subsp. 暴发。这些鹅群由 2-3 周龄的 160-1450 只小鹅组成，死亡率增加。临床症状的特征是严重的中枢神经系统症状，即腿部划动和斜颈。剖检调查显示肝炎、脾炎和腹腔内液体量少。肝脏质地脆弱，有白色坏死区。脾脏也有这种情况。大脑中没有肉眼可见的病变。随后进行的细菌学调查，通过基质辅助激光解吸飞行时间质谱法进行细菌鉴定和部分 16S rRNA 区域的系统发育分析，显示 subsp. 存在于心脏、肝脏、脾脏和大脑中。组织学调查显示，肝脏和脾脏样本中存在多灶性坏死，同时有单核细胞和异嗜性粒细胞浸润。此外，在病变中可以鉴定出球菌。鹅群的脑组织样本中没有观察到组织病理学变化，除了一只鹅的脑组织样本中存在血管内球菌积聚。抗生素敏感性试验显示所有菌株的图谱相同，对青霉素、头孢菌素、氯霉素、亚胺培南和泰乐菌素敏感。然而，对喹诺酮类、氨基糖苷类、四环素类和磺胺甲恶唑-甲氧苄啶类药物的耐药性也存在，这些药物常用于治疗革兰氏阳性菌感染。
