School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China.
School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China.
Environ Res. 2021 Nov;202:111786. doi: 10.1016/j.envres.2021.111786. Epub 2021 Jul 30.
To evaluate the simultaneous nitrification and denitrification (SND) performance of the aeration solid-phase denitrification (SPD) process and improve the operating efficiency, aeration SPD process using polybutanediol succinate as carbon source was optimized and the process was bioaugmented with heterotrophic nitrification-aerobic denitrification bacteria for the treatment of real wastewater. The results showed that after bioaugmentation, the total nitrogen removal efficiency of the aeration SPD process increased by 50.46 % under condition of dissolved oxygen (DO) 3 mg/L. According to Illumina MiSeq sequencing and correlation analyses, the microbial community can perform SND under the conditions of DO 5 mg and HRT 6 h, but is susceptible to DO. Bioaugmentation mainly affected the carbon source metabolic network with heterotrophic bacteria Methyloversatilis, Thiothrix, and norank_Lentimicrobiaceae as nodes to change the community structure, thereby improving the performance of the functional microbial community. Kyoto Encyclopedia of Genes and Genomes analysis suggested that narB, narG, narH, nirK and narI were the key genes involved in the response to bioaugmentation. This work provides new insights for the application of the SPD process in wastewater treatment.
为了评估曝气固相反硝化（SPD）工艺的同步硝化反硝化（SND）性能并提高运行效率，采用丁二酸聚酯作为碳源对曝气 SPD 工艺进行了优化，并通过添加异养硝化-好氧反硝化细菌对实际废水进行了生物强化。结果表明，生物强化后，曝气 SPD 工艺在溶解氧（DO）为 3mg/L 的条件下，总氮去除效率提高了 50.46%。根据 Illumina MiSeq 测序和相关性分析，微生物群落可以在 DO 为 5mg 和水力停留时间（HRT）为 6h 的条件下进行 SND，但易受 DO 影响。生物强化主要通过以异养细菌 Methyloversatilis、Thiothrix 和 norank_Lentimicrobiaceae 为节点的碳源代谢网络来影响群落结构，从而改善功能微生物群落的性能。京都基因与基因组百科全书分析表明，narB、narG、narH、nirK 和 narI 是对生物强化反应的关键基因。这项工作为 SPD 工艺在废水处理中的应用提供了新的见解。
