Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Department of Genome Sciences, University of Washington, Seattle, Washington, USA; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, USA.
Mol Cell Proteomics. 2021;20:100129. doi: 10.1016/j.mcpro.2021.100129. Epub 2021 Jul 30.
Post-translational modification (PTM) of proteins allows cells to regulate protein functions, transduce signals and respond to perturbations. PTMs expand protein functionality and diversity, which leads to increased proteome complexity. PTM crosstalk describes the combinatorial action of multiple PTMs on the same or on different proteins for higher order regulation. Here we review how recent advances in proteomic technologies, mass spectrometry instrumentation, and bioinformatics spurred the proteome-wide identification of PTM crosstalk through measurements of PTM sites. We provide an overview of the basic modes of PTM crosstalk, the proteomic methods to elucidate PTM crosstalk, and approaches that can inform about the functional consequences of PTM crosstalk.
蛋白质的翻译后修饰 (PTM) 使细胞能够调节蛋白质功能、转导信号并对干扰做出反应。PTM 扩展了蛋白质的功能和多样性，从而增加了蛋白质组的复杂性。PTM 串扰描述了多种 PTM 在同一或不同蛋白质上的组合作用，以实现更高阶的调控。在这里，我们回顾了蛋白质组学技术、质谱仪器和生物信息学的最新进展如何通过测量 PTM 位点来促进 PTM 串扰的全蛋白质组鉴定。我们提供了 PTM 串扰的基本模式概述、阐明 PTM 串扰的蛋白质组学方法，以及可以提供 PTM 串扰功能后果信息的方法。
