State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Nanjing Stomatological Hospital, Medical school of Nanjing University, Nanjing, China.
Oral Dis. 2023 Jan;29(1):254-264. doi: 10.1111/odi.13989. Epub 2021 Aug 13.
To investigate the effects of different lactoferrin concentrations on mid-palatal suture bone remodeling during palatal expansion and relapse in rats.
Thirty-two 5-week-old male Wistar rats were randomly divided into four groups: EO (expansion only), E+LF1 (expansion plus 10 mg/kg/day daily LF), E+LF2 (expansion plus 100 mg/kg/day daily LF), and E+LF3 (expansion plus 1 g/kg/day daily LF). Thereafter, micro-computed tomography and micro-morphology of the mid-palatal suture were analyzed on day 7 and day 14, respectively.
The arch widths were increased in all the four groups after expansion, and there was no significant difference among them on day 7. After relapse, however, the arch width in the E+LF3 group was significantly larger compared with EO group. In E+LF3 group and E+LF2 group, new bone formation and osteoblast number were enhanced with up-regulated expression of osteocalcin and collagen type I, while the expression of cathepsin K-positive cells was downregulated in E+LF3 group.
Lactoferrin gavage administration might increase the stability of palatal expansion and reduce relapse in a concentration-dependent manner by enhancing bone formation and inhibiting resorption. LF administration may be promising for optimizing the maxillary expansion outcome.
研究不同乳铁蛋白浓度对大鼠腭中缝骨改建在扩弓及复发过程中的影响。
32 只 5 周龄雄性 Wistar 大鼠随机分为 4 组：E 组（仅扩弓）、E+LF1 组（扩弓加 10mg/kg/天乳铁蛋白）、E+LF2 组（扩弓加 100mg/kg/天乳铁蛋白）和 E+LF3 组（扩弓加 1g/kg/天乳铁蛋白）。分别于扩弓后第 7 天及第 14 天进行微焦点 CT 扫描及中缝骨的形态学分析。
扩弓后 4 组的牙弓宽度均增加，第 7 天 4 组间无显著差异。但复发后，E+LF3 组的牙弓宽度显著大于 EO 组。在 E+LF3 组和 E+LF2 组中，骨形成和成骨细胞数量增加，骨钙素和 I 型胶原表达上调，而 E+LF3 组中组织蛋白酶 K 阳性细胞的表达下调。
乳铁蛋白灌胃可能通过增加骨形成和抑制吸收，以浓度依赖的方式增加腭中缝骨改建的稳定性，减少复发。乳铁蛋白的应用可能有助于优化上颌骨扩弓的效果。
