Bellin M E, Ax R L
Department of Dairy Science, University of Wisconsin-Madison.
Adv Exp Med Biol. 1987;219:731-5. doi: 10.1007/978-1-4684-5395-9_51.
The two glycosaminoglycans found in FF, DS and HS were isolated from fluid of atretic and non-atretic bovine follicles and simultaneously separated by anion exchange high performance liquid chromatography. Heparan sulfate eluted with the apex of the peak at .071 M NaCl. Dermatan sulfate eluted with the apex of the peak at .353 M NaCl. The concentrations of DS and HS were greater in atretic follicles compared to non-atretic follicles and decreased as follicles size increased, as previously reported. The proportion of DS was approximately 90% of the total glycosaminoglycan concentration of all FF samples. The ratio of galactosamine to glucosamine in DS increased as follicle size increased, being 3.44 +/- .35, 7.65 +/- 2.4, and 15.9 +/- 3.8 (P less than .05) for small, medium and large follicles, respectively.
在闭锁和未闭锁牛卵泡液中发现的两种糖胺聚糖，从闭锁和未闭锁牛卵泡的卵泡液中分离出来，并同时通过阴离子交换高效液相色谱法进行分离。硫酸乙酰肝素在0.071M氯化钠时以峰尖洗脱。硫酸皮肤素在0.353M氯化钠时以峰尖洗脱。如先前报道，与未闭锁卵泡相比，闭锁卵泡中硫酸皮肤素和硫酸乙酰肝素的浓度更高，且随着卵泡大小增加而降低。硫酸皮肤素的比例约占所有卵泡液样本中总糖胺聚糖浓度的90%。硫酸皮肤素中半乳糖胺与葡萄糖胺的比例随着卵泡大小增加而增加，小、中、大卵泡分别为3.44±0.35、7.65±2.4和15.9±3.8（P<0.05）。
