Sinel'nikov G E, Preger S M, Muzafarova N Kh
Zh Mikrobiol Epidemiol Immunobiol. 1978 Jan(1):123-7.
It was shown that purified and concentrated by the "Diaferm" method antibotulin sera from horse and cattle blood failed to differ by anaphylactogenic properties; at the same time in sensitization of the organism to protein of one animal species the use of the sera of another species provided a lesser reactogenicity of the preparation. The antigenic activity of the purified and concentrated sera from the blood or horses and cows in testing on rabbits was identical, but in response to cow alpha-globulin the animals responsed by a more intensive production of precipitins. The activity of cow and horse antibotulin serum (determined by the rate and stability of their association with the corresponding toxin) proved to be identical.
结果表明，用“Diaferm”方法纯化浓缩的马和牛血液抗肉毒血清在变应原性方面没有差异；同时，在使机体对一种动物物种的蛋白质致敏时，使用另一种物种的血清可使制剂的反应原性降低。从马和牛血液中纯化浓缩的血清在兔身上进行检测时，其抗原活性相同，但在针对牛α-球蛋白时，动物产生沉淀素的反应更强烈。牛和马抗肉毒血清的活性（由它们与相应毒素结合的速率和稳定性决定）被证明是相同的。
