Sakaki Junichi R, Melough Melissa M, Yang Cathy Z, Provatas Anthony A, Perkins Christopher, Chun Ock K
Department of Nutritional Sciences, University of Connecticut, Storrs, CT, USA.
CertiChem Inc., Austin, TX, USA.
Curr Res Toxicol. 2021 May 18;2:210-216. doi: 10.1016/j.crtox.2021.05.003. eCollection 2021.
Coffee brewed from capsule machines may contain estrogenic chemicals migrated from plastic, but the estrogenic activity of capsule coffee has not been evaluated. This study evaluated the estrogenic activity of capsule coffee using the VM7Luc4E2 estrogen receptor transcriptional activation assay. Estrogenic potentials of six capsule coffee samples were calculated using relative maximum amplitude response of E2 (>15%RME2 indicative of estrogenic activity) and estradiol equivalent factor (EEF). Estrogenic chemical content was determined using ultra-performance liquid chromatography with tandem mass spectrometry. All capsule coffee samples possessed estrogenic activity (48-56%RME2). EEFs were 6-7 orders of magnitude lower than that of E2, (1.2 × 10-1.7 × 10), indicating substantially weaker estrogenic potencies. Bisphenol A, bisphenol F, benzophenone, 4-nonylphenol, dibutyl phthalate, and dimethyl terephthalate were detected in capsule coffee. Capsule coffee exhibited estrogenic activity in vitro, and its estrogenic chemical content is likely driving its estrogenicity, warranting further investigations to fully understand the degree to which they are related and to predict the estrogenic potential based on the concentration of estrogenic chemicals.
胶囊咖啡机冲泡的咖啡可能含有从塑料中迁移出来的具有雌激素活性的化学物质，但胶囊咖啡的雌激素活性尚未得到评估。本研究使用VM7Luc4E2雌激素受体转录激活试验评估了胶囊咖啡的雌激素活性。使用E2的相对最大幅度响应（>15%RME2表示具有雌激素活性）和雌二醇当量因子（EEF）计算了六个胶囊咖啡样品的雌激素潜力。使用超高效液相色谱串联质谱法测定了具有雌激素活性的化学物质含量。所有胶囊咖啡样品均具有雌激素活性（48 - 56%RME2）。EEF比E2低6 - 7个数量级（1.2×10 - 1.7×10），表明其雌激素效力明显较弱。在胶囊咖啡中检测到了双酚A、双酚F、二苯甲酮、4 - 壬基酚、邻苯二甲酸二丁酯和对苯二甲酸二甲酯。胶囊咖啡在体外表现出雌激素活性，其具有雌激素活性的化学物质含量可能是其雌激素性的驱动因素，需要进一步研究以充分了解它们之间的关联程度，并根据具有雌激素活性的化学物质浓度预测雌激素潜力。
