Brodie H A, Chole R A
Department of Otolaryngology-Head and Neck Surgery, University of California, Davis 95616.
Am J Otolaryngol. 1987 Sep-Oct;8(5):342-50. doi: 10.1016/s0196-0709(87)80053-4.
An immunohistologic study was performed on temporal bones from 30 LP/J mice and 17 CBA/J mice to assess the role of immunologic injury in the pathogenesis of dysplastic bony lesions in LP/J mice. Temporal bones were harvested from animals aged 2 to 31 months to evaluate the progression of the disease. As early as 2 months of age, before the onset of bony lesions, the tympanic cavities frequently contained small effusions coating the ossicles and otic capsules that were demonstrated to contain immunoglobulins and pockets of macrophages. Later in the course of the disease, bony lesions grossly and histologically similar to human otosclerosis developed, which stained for immunoglobulins. No similar bony lesions, effusions, cellular infiltrates, or staining for immunoglobulins was detected in the control animals, even in the presence of acute otitis media. This study suggests a role of immunologic injury in the pathogenesis of dysplastic bony lesions in LP/J mice.
对30只LP/J小鼠和17只CBA/J小鼠的颞骨进行了免疫组织学研究，以评估免疫损伤在LP/J小鼠发育异常性骨病变发病机制中的作用。从2至31月龄的动物中采集颞骨，以评估疾病的进展。早在2月龄，在骨病变出现之前，鼓室中经常含有覆盖听小骨和耳囊的小积液，经证实这些积液含有免疫球蛋白和巨噬细胞聚集区。在疾病后期，出现了大体和组织学上与人类耳硬化症相似的骨病变，这些病变免疫球蛋白染色呈阳性。在对照动物中，即使存在急性中耳炎，也未检测到类似的骨病变、积液、细胞浸润或免疫球蛋白染色。这项研究表明免疫损伤在LP/J小鼠发育异常性骨病变的发病机制中起作用。
