采用邻苯二甲醛柱前衍生化法对1-氨基环丙烷-1-羧酸进行高效液相色谱分析。
High-performance liquid chromatography analysis of 1-aminocyclopropane-1-carboxylic acid using o-phthaldialdehyde precolumn derivatization.
作者信息
Bushey D F, Law D M, Davies P J
机构信息
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
出版信息
Anal Biochem. 1987 Nov 15;167(1):31-6. doi: 10.1016/0003-2697(87)90130-8.
PMID:3434798
Abstract
A simple, rapid, direct method for the HPLC analysis of 1-aminocyclopropane-1-carboxylic acid (ACC) as its o-phthaldialdehyde derivative is described. The method is sensitive to about 1 pmol and can be used on plant tissue extracts with no cleanup. It will prove valuable in plant extracts where the chemical conversion of ACC in the tissue extracts to ethylene is variable, or when analyzing the specific radioactivity of ACC produced from radiolabeled precursors.
摘要
本文描述了一种简单、快速、直接的高效液相色谱（HPLC）分析方法，用于分析1-氨基环丙烷-1-羧酸（ACC）的邻苯二甲醛衍生物。该方法的灵敏度约为1皮摩尔，可直接用于未经净化处理的植物组织提取物。在植物提取物中，当组织提取物中ACC向乙烯的化学转化存在差异时，或者在分析由放射性标记前体产生的ACC的比放射性时，该方法将具有重要价值。
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