Department of Anatomy and Embryology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Toxicol Mech Methods. 2022 Jan;32(1):58-66. doi: 10.1080/15376516.2021.1965275. Epub 2021 Aug 22.
Human exposure to nanoparticles became unavoidable secondary to their massive involvement in a multitude of industrial applications. Zinc oxide nanoparticles (ZnONPs) are one of the most commonly used metal oxide nanoparticles in biological applications. Naringenin (NAR), a citrus-derived flavonoid, has favorable biological properties that promote human health. The present study was carried out to investigate the possible defensive role of NAR versus ZnONPs provoked hepatic injury in rats through an evaluation of liver enzymes, hepatic biomarkers of oxidative stress, inflammatory process, apoptotic cell death along with histopathological examination of liver tissue. Therefore, 32 adult rats were randomly divided into four equal groups as control, NAR, ZnONPs and co-treated ZnONPs with NAR groups. All treatments were administered for 14 days. Our results showed that ZnONPs induced hepatic injury as documented by the marked increased in hepatic enzymes activities, disturbed hepatic oxidant/antioxidant balance, increased hepatic inflammatory reactions, in addition to, extensive hepatic morphological alterations, marked collagen fibers accumulation as well as overexpression of apoptotic BAX and the noticeable intensified positive nuclear staining for nuclear factor Kabba-b in hepatic tissues. Concurrent NAR supplement to ZnONPs- treated rats significantly declined liver enzymes activities, restored oxidant/antioxidant balance, reversed inflammation, induced fewer collagen fibers accumulation, and antagonized BAX-mediated apoptotic cell death in hepatic tissues. We concluded that concurrent NAR supplement to ZnONPs treated rats improved hepatic function and structure by its antioxidant, anti-inflammatory and antiapoptotic potentials.
由于纳米粒子在众多工业应用中的大量使用，人类接触纳米粒子已不可避免。氧化锌纳米粒子（ZnONPs）是生物应用中最常用的金属氧化物纳米粒子之一。柚皮素（NAR），一种源自柑橘的类黄酮，具有促进人类健康的良好生物学特性。本研究旨在通过评估肝脏酶、肝氧化应激生物标志物、炎症过程、细胞凋亡以及肝组织的组织病理学检查，研究 NAR 对 ZnONPs 引起的大鼠肝损伤的可能防御作用。因此，将 32 只成年大鼠随机分为 4 组，分别为对照组、NAR 组、ZnONPs 组和 NAR 与 ZnONPs 共同处理组。所有治疗均持续 14 天。我们的结果表明，ZnONPs 诱导了肝损伤，表现为肝脏酶活性显著增加，肝氧化应激/抗氧化平衡失调，肝炎症反应增强，此外，肝组织形态学改变广泛，胶原纤维大量积累，以及 BAX 表达上调和肝组织中核因子 Kappa-B 的阳性核染色明显增强。同时给予 NAR 补充 ZnONPs 处理的大鼠，肝脏酶活性显著下降，氧化应激/抗氧化平衡得到恢复，炎症得到逆转，胶原纤维积累减少，BAX 介导的细胞凋亡减少。我们得出结论，同时给予 NAR 补充 ZnONPs 处理的大鼠通过其抗氧化、抗炎和抗凋亡作用改善了肝功能和结构。
