Pascoe D R, Pascoe R R, Hughes J P, Stabenfeldt G H, Kindahl H
Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
Am J Vet Res. 1987 Nov;48(11):1594-9.
One hundred mares carrying twin conceptuses between gestation days (GD) 12 and 30 were assigned to 4 groups. Group-1 mares (n = 20) were given a placebo (sterile saline solution, IV, and sesame oil, IM). Group-2 mares (n = 32) were given a single dose of progesterone (625 mg, IM) in sesame oil. Group-3 mares (n = 28) were given multiple progesterone treatments (625 mg, IM) at 6-day intervals until GD 42. Group-4 mares (n = 20) were given a single treatment of flunixin meglumine (500 mg, IV) and progesterone (625 mg, IM). Each group was further assigned to equal subgroups A and B according to the age of the embryos. In subgroup A, with embryos at GD 12 to 16, embryonic vesicle rupture was performed after the selected conceptus was moved to the tip of a uterine horn. In subgroup B, with embryos at GD 17 to 30, embryonic vesicle rupture was performed on the selected conceptus in situ. An additional 6 mares carrying single conceptuses (GD 12, 14, 16, 20, 25, and 30) were used as subjects for sham embryonic vesicle rupture. Both rupture techniques usually caused the release of prostaglandin (PG) F2 alpha for up to 90 minutes. Amounts of PGF2 alpha were directly correlated with the pressure required to effect embryonic vesicle rupture. Sham embryonic vesicle rupture attempts caused small PGF2 alpha releases after each manipulation, which rapidly returned to basal values. All 6 mares were still pregnant at GD 42. Flunixin meglumine inhibited PGF2 alpha release after embryonic vesicle rupture, regardless of the technique.(ABSTRACT TRUNCATED AT 250 WORDS)
100匹怀有双胎、孕期在妊娠第12天至30天之间的母马被分为4组。第1组母马（n = 20）给予安慰剂（无菌生理盐水溶液，静脉注射，芝麻油，肌肉注射）。第2组母马（n = 32）给予单剂量孕酮（625毫克，肌肉注射），溶于芝麻油中。第3组母马（n = 28）每隔6天给予多次孕酮治疗（625毫克，肌肉注射），直至妊娠第42天。第4组母马（n = 20）给予单剂量氟尼辛葡甲胺（500毫克，静脉注射）和孕酮（625毫克，肌肉注射）。根据胚胎年龄，每组进一步分为相等的亚组A和B。在亚组A中，胚胎处于妊娠第12天至16天，在选定的孕体移至子宫角尖端后进行胚泡破裂。在亚组B中，胚胎处于妊娠第17天至30天，在选定的孕体原位进行胚泡破裂。另外6匹怀有单胎（妊娠第12天、14天、16天、20天、25天和30天）的母马用作假胚泡破裂的实验对象。两种破裂技术通常都会导致前列腺素（PG）F2α释放长达90分钟。PGF2α的释放量与导致胚泡破裂所需的压力直接相关。假胚泡破裂尝试在每次操作后导致少量PGF2α释放，随后迅速恢复到基础值。所有6匹母马在妊娠第42天时仍处于妊娠状态。无论采用何种技术，氟尼辛葡甲胺均可抑制胚泡破裂后PGF2α的释放。（摘要截选至250字）
