Denner J, Ehm I, Jandrig B, Friese D
Central Institute for Cancer Research, Robert-Rössle-Institute, Academy of Sciences, GDR Berlin-Buch.
Arch Geschwulstforsch. 1987;57(6):463-74.
Immortalized mouse NIH 3T3 cells were transformed by gene transfer of DNA isolated from a human bladder tumor cell line and plasmids containing an activated human Ha-ras oncogene insert. For gene transfer the calcium-phosphate co-precipitation method was used. Transformation was evaluated by morphological focus formation, growth in soft agar and tumor development in nude mice. In addition, immortalized rat FR 3T3 cells were transformed by Ha-ras, too. The co-transfer of ras and myc oncogenes did not enhance focus formation in FR 3T3 cells.
永生化小鼠NIH 3T3细胞通过从人膀胱肿瘤细胞系分离的DNA以及含有激活的人Ha-ras癌基因插入片段的质粒进行基因转移而被转化。基因转移采用磷酸钙共沉淀法。通过形态学集落形成、软琼脂中的生长以及裸鼠体内肿瘤发展来评估转化情况。此外，永生化大鼠FR 3T3细胞也通过Ha-ras进行了转化。ras和myc癌基因的共转移并未增强FR 3T3细胞中的集落形成。
