污泥堿性发酵生产挥发性脂肪酸过程中细菌的生存策略：细菌群落生理特性、时间演变和空间分布的研究。
Bacterial survival strategies in sludge alkaline fermentation for volatile fatty acids production: Study on the physiological properties, temporal evolution and spatial distribution of bacterial community.
机构信息
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
出版信息
Bioresour Technol. 2021 Nov;340:125701. doi: 10.1016/j.biortech.2021.125701. Epub 2021 Jul 31.
This study investigated the dynamics of ATP synthase activity, phospholipid fatty acid (PLFA) profile, and temporal evolution and spatial distribution of bacterial community to analyze bacterial survival strategies in sludge alkaline fermentation (SAF) for volatile fatty acids (VFAs) production. The results revealed a significant increase in ATP synthase activity at pH 9 and 10 (p < 0.05), which could contribute to proton entry into cells and benefit bacterial survival. PLFA analysis indicated that the unsaturated fatty acids content increased with the increase of pH. Firmicutes were the dominant microorganisms in the running stage of the pH 10 reactor (35.81-62.34%) and might have been the key microbes that influenced VFAs production. Further analysis of the spatial distribution of microbial community suggested that Firmicutes mainly lived inside flocs during SAF. These findings provide an understanding for bacterial survival strategies in SAF, which could help to develop methods to further improve VFAs yield.
本研究调查了 ATP 合酶活性、磷脂脂肪酸 (PLFA) 谱、细菌群落的时空演变和空间分布，以分析污泥碱性发酵 (SAF) 中细菌的生存策略，以生产挥发性脂肪酸 (VFAs)。结果表明，在 pH 值为 9 和 10 时，ATP 合酶活性显著增加（p < 0.05），这有助于质子进入细胞并有利于细菌生存。PLFA 分析表明，不饱和脂肪酸含量随 pH 值的升高而增加。厚壁菌门是 pH 值为 10 的反应器运行阶段的优势微生物（35.81-62.34%），可能是影响 VFAs 生产的关键微生物。对微生物群落空间分布的进一步分析表明，厚壁菌门主要在 SAF 过程中生活在絮体内部。这些发现为 SAF 中细菌的生存策略提供了深入的了解，有助于开发进一步提高 VFAs 产量的方法。
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