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犬回肠造口术的研究:功能方面、光学显微镜及扫描电子显微镜观察

Study of ileostomy in dogs, functional aspects, optic and scanning electronic microscopy.

作者信息

Mattos M P, Lázaro da Silva A

机构信息

School of Veterinary Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.

出版信息

Arq Gastroenterol. 1986 Jul-Sep;23(3):159-68.

PMID:3435265
Abstract

Containing ileostomy has been a permanent aim of researchers. Different technics for containing in the ileal derivations has been proposed, very little filled the requisites, but no one reached the desired aim, and the problem continues unsolved. The authors, based in experimental study in two groups of dogs submitted to ileostomy, with and without preservation of ileal pylorus, observed the following parameters: weight dog clinical evaluation of animals (diarrhea, infection of surgical wound and malnutrition) volume of ileal excretions electrolytes (Na+, Cl- and K+) pH of ileal excretions and deaths. After sacrificing the animals we analyzed on scanning electronic microscopy, the presence of mucus, clearness of mucosa surface, general aspect of terminal ileum mucosa, and on optic microscopy, neutrophilic infiltration, increasing of number of goblet cells, hypotrophy of villosities and determination of ileal villosities height. After statistic analysis of results we concluded that ileostomy with preservation of ileal pylorus, markedly attenuated the variable of control in the experiment. Conservation of ileal pylorus, consequently has an effective action on the control of ileal leakage, protecting its mucosa from serious histopathologic changes.

摘要

控制回肠造口术一直是研究人员的一个长期目标。人们提出了不同的技术来控制回肠引流,但很少有技术满足要求,而且没有一种技术达到预期目标,这个问题仍然没有解决。作者基于对两组接受回肠造口术的狗进行的实验研究,一组保留回肠幽门,另一组不保留,观察了以下参数:狗的体重、动物的临床评估(腹泻、手术伤口感染和营养不良)、回肠排泄物的体积、电解质(Na+、Cl-和K+)、回肠排泄物的pH值以及死亡情况。在处死动物后,我们通过扫描电子显微镜分析了黏液的存在、黏膜表面的清晰度、回肠末端黏膜的总体外观,以及通过光学显微镜分析了中性粒细胞浸润、杯状细胞数量增加、绒毛萎缩和回肠绒毛高度的测定。对结果进行统计分析后,我们得出结论,保留回肠幽门的回肠造口术显著减轻了实验中的对照变量。因此,保留回肠幽门对控制回肠渗漏有有效作用,保护其黏膜免受严重的组织病理学变化影响。

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