Gellerich F N, Kunz W
Institut für Biochemie der Medizinischen Akademie, Magdeburg, GDR.
Biomed Biochim Acta. 1987;46(8-9):S545-8.
By means of reconstituted systems consisting in rat heart mitochondria and pyruvate kinase it was shown, that the mitochondrial ATP-production is remarkable increased if ADP is produced by enzymes localized in the mitochondrial intermembrane space as creatine kinase or adenylate kinase. This result and different amount of inhibition by atractyloside or carboxyatractyloside of hexokinase- and creatine kinase stimulated respiration further support the concept of dynamic compartmentation of adenine nucleotides in the mitochondrial intermembrane space.
通过由大鼠心脏线粒体和丙酮酸激酶组成的重组系统表明，如果二磷酸腺苷（ADP）由位于线粒体外膜间隙的酶（如肌酸激酶或腺苷酸激酶）产生，线粒体的三磷酸腺苷（ATP）生成会显著增加。这一结果以及苍术苷或羧基苍术苷对己糖激酶和肌酸激酶刺激的呼吸的不同抑制程度，进一步支持了腺嘌呤核苷酸在线粒体外膜间隙动态分隔的概念。
