Singh Mukti, Becker Mark, Godwin Alan R F, Baldock Clair
Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PT, UK.
Matrix Biol Plus. 2021 Jul 7;12:100078. doi: 10.1016/j.mbplus.2021.100078. eCollection 2021 Dec.
Elastic tissues owe their functional properties to the composition of their extracellular matrices, particularly the range of extracellular, multidomain extensible elastic fibre and microfibrillar proteins. These proteins include elastin, fibrillin, latent TGFβ binding proteins (LTBPs) and collagens, where their biophysical and biochemical properties not only give the matrix structural integrity, but also play a vital role in the mechanisms that underlie tissue homeostasis. Thus far structural information regarding the structure and hierarchical assembly of these molecules has been challenging and the resolution has been limited due to post-translational modification and their multidomain nature leading to flexibility, which together result in conformational and structural heterogeneity. In this review, we describe some of the matrix proteins found in elastic fibres and the new emerging techniques that can shed light on their structure and dynamic properties.
弹性组织的功能特性归因于其细胞外基质的组成，特别是细胞外多结构域可伸展弹性纤维和微原纤维蛋白的种类。这些蛋白质包括弹性蛋白、原纤蛋白、潜伏转化生长因子β结合蛋白（LTBPs）和胶原蛋白，它们的生物物理和生化特性不仅赋予基质结构完整性，而且在组织稳态的潜在机制中起着至关重要的作用。到目前为止，关于这些分子的结构和层次组装的结构信息一直具有挑战性，并且由于翻译后修饰及其多结构域性质导致的灵活性，分辨率受到限制，这共同导致构象和结构异质性。在这篇综述中，我们描述了在弹性纤维中发现的一些基质蛋白以及能够揭示其结构和动态特性的新出现的技术。
