Atrazhev A M, Diatkina N B, Rozovskaia T A, Aleksandrova L A, Chidzhavadze Z G
Bioorg Khim. 1987 Oct;13(10):1375-81.
Incorporation of 2'-deoxyribonucleotide 5'-triphosphate derivatives, chemically modified both in the base and at 3'-position, into DNA by four different DNA polymerases was investigated. It is shown that 3'-azido- and 3'-amino-2',3'-dideoxy-(E)-5-(2-bromovinyl)-uridine 5'-triphosphates effectively terminate DNA synthesis catalyzed by E. coli DNA polymerase I, rat liver DNA polymerase beta, and AMV reverse transcriptase. Calf thymus DNA polymerase alpha incorporates only the 3'-amino derivative. DNA polymerases I and beta catalyse DNA synthesis in the presence of beta-D-(2'-deoxyribofuranosyl)-1-benzimidazol 5'-triphosphate, inserting the corresponding monophosphate in place of -dGTP, whereas 3'-substituted analogues of this compound were inactive in the reactions.
研究了四种不同的DNA聚合酶将在碱基和3'-位均经化学修饰的2'-脱氧核糖核苷酸5'-三磷酸衍生物掺入DNA的情况。结果表明，3'-叠氮基-和3'-氨基-2',3'-二脱氧-(E)-5-(2-溴乙烯基)-尿苷5'-三磷酸能有效终止由大肠杆菌DNA聚合酶I、大鼠肝脏DNA聚合酶β和禽成髓细胞瘤病毒逆转录酶催化的DNA合成。小牛胸腺DNA聚合酶α仅掺入3'-氨基衍生物。DNA聚合酶I和β在β-D-(2'-脱氧呋喃核糖基)-1-苯并咪唑5'-三磷酸存在的情况下催化DNA合成，将相应的单磷酸插入取代-dGTP，而该化合物的3'-取代类似物在反应中无活性。
