CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory for Marine Biology and Biotechnology, Pilot Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Mar Drugs. 2021 Jul 13;19(7):392. doi: 10.3390/md19070392.
β-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms owing to the lack of omics-based information. In this study, we first compared the chitin content of three representative species. Cell wall glycosidic linkage analysis and chitin/chitosan staining assays showed that was an appropriate candidate chitin producer. A full-length (FL) transcriptome of was obtained via PacBio sequencing. In total, the FL transcriptome comprised 23,362 annotated unigenes, 710 long non-coding RNAs (lncRNAs), 363 transcription factors (TFs), 3113 alternative splicing (AS) events and 3295 simple sequence repeats (SSRs). More specifically, 234 genes related to chitin metabolism were identified and the complete biosynthetic pathways of chitin and chitosan were explored. The information presented here will facilitate molecular research and the exploitation of β-chitin-derived high-value enzymes and products.
由于其结构由几丁质的平行链组成、性质以及硅藻的高丰度，由硅藻产生的β-几丁质预计将具有重要的经济和生态价值。然而，由于缺乏基于组学的信息，很少有研究对硅藻中的几丁质相关基因进行功能表征。在这项研究中，我们首先比较了三种代表性物种的几丁质含量。细胞壁糖苷键分析和几丁质/壳聚糖染色试验表明， 是合适的几丁质产生候选物。通过 PacBio 测序获得了 的全长 (FL) 转录组。总共，FL 转录组包括 23362 个注释的 unigenes、710 个长非编码 RNA（lncRNA）、363 个转录因子（TF）、3113 个可变剪接（AS）事件和 3295 个简单序列重复（SSR）。更具体地，鉴定了 234 个与几丁质代谢相关的基因，并探索了几丁质和壳聚糖的完整生物合成途径。这里提供的信息将有助于 的分子研究和开发β-几丁质衍生的高价值酶和产品。
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