十二烷基硫酸钠（SDS）和2-巯基乙醇对人胃黏液凝胶超分子结构的影响。
The effect of SDS and 2-mercaptoethanol on the supramolecular structure of human gastric mucus gel.
作者信息
Gindzieński A, Zwierz K
机构信息
Research Laboratory of Biochemistry, Institute of Chemistry, Medical Academy, Mickiewicza, Poland.
出版信息
Biochem Med Metab Biol. 1987 Dec;38(3):347-54. doi: 10.1016/0885-4505(87)90099-5.
The solubility of freshly obtained human gastric mucus gel was investigated using solutions of Tris buffer, urea, SDS, and 2-mercaptoethanol. The best solubility was achieved in SDS solution, whereas 2-mercaptoethanol did not solubilize mucus gel. The degree of mucus depolymerization with the solubilizers was evaluated using light scattering and viscosity measurements. In mucus fractions, neutral carbohydrate, protein, unsaturated fatty acids, and cholesterol were determined. Some aspects of mucus composition and structure are discussed.
使用Tris缓冲液、尿素、SDS和2-巯基乙醇溶液研究了新鲜获取的人胃黏液凝胶的溶解性。在SDS溶液中溶解性最佳，而2-巯基乙醇不能溶解黏液凝胶。使用光散射和粘度测量评估了增溶剂对黏液的解聚程度。测定了黏液组分中的中性碳水化合物、蛋白质、不饱和脂肪酸和胆固醇。讨论了黏液组成和结构的一些方面。
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