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一种来自黄守瓜(双翅目:蚤蝇科)的新型双尾病毒,黄守瓜是甘蔗的一种害虫。

A new dicistro-like virus from soldier fly, Inopus flavus (Diptera: Stratiomyidae), a pest of sugarcane.

机构信息

School of Biological Sciences, The University of Queensland, Brisbane, Saint Lucia, QLD, 4072, Australia.

出版信息

Arch Virol. 2021 Oct;166(10):2841-2846. doi: 10.1007/s00705-021-05171-2. Epub 2021 Aug 6.

Abstract

Native Australian soldier flies, Inopus spp. (Diptera: Stratiomyidae), are agricultural pests of economic importance to the sugarcane industry. A screen of the salivary gland transcriptome of Inopus flavus (James) revealed the presence of viral RNA belonging to a potentially novel member of the family Dicistroviridae. The complete genome sequence consists of 9793 nucleotides with two open reading frames. The genome includes two potential internal ribosomal entry sites (IRESs): one within the 5' UTR and the other in the intergenic region (IGR). Virus particles purified from infected larvae and visualised by electron microscopy were found to be icosahedral, non-enveloped, and 30 nm in diameter.

摘要

本土澳大利亚士兵蝇属(双翅目:Stratiomyidae)是甘蔗产业的经济重要农业害虫。对 Inopus flavus(James)唾液腺转录组的筛选显示存在属于 Dicistroviridae 家族潜在新成员的病毒 RNA。完整基因组序列由 9793 个核苷酸组成,包含两个开放阅读框。基因组包含两个潜在的内部核糖体进入位点(IRES):一个在 5'UTR 内,另一个在基因间区(IGR)内。从感染幼虫中纯化并通过电子显微镜观察到的病毒颗粒为二十面体、无包膜、直径 30nm。

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