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硝酸盐触发的磷酸化蛋白质组变化和一个调节根系结构的 PIN2 磷酸化位点。

Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture.

机构信息

Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile.

FONDAP Center for Genome Regulation, ANID - Millennium Science Initiative Program - Millennium Institute for Integrative Biology (iBio), Santiago, Chile.

出版信息

EMBO Rep. 2021 Sep 6;22(9):e51813. doi: 10.15252/embr.202051813. Epub 2021 Aug 6.

DOI:10.15252/embr.202051813
PMID:34357701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8447600/
Abstract

Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate.

摘要

硝酸盐可引起全基因组基因表达的变化，从而影响代谢、生理、植物生长和发育。为了鉴定植物硝酸盐响应中涉及的新成分，我们通过液相色谱-串联质谱联用技术分析了拟南芥根在硝酸盐处理下的磷酸化蛋白质组。176 种磷酸化蛋白在硝酸盐处理后 5 或 20 分钟时显示出明显的变化。在 5 分钟时鉴定出的蛋白包括激酶或转录因子等信号成分。相比之下，在 20 分钟时鉴定出的蛋白与转运体活性或激素代谢功能等有关。与野生型植物相比，硝酸盐转运蛋白 1.1 (NRT1.1) 突变体植物的磷酸化图谱发生了显著改变，证实了其在涉及磷酸化变化的硝酸盐信号通路中的关键作用。综合生物信息学分析突出了生长素运输作为硝酸盐信号在翻译后水平调节的一个重要机制。我们验证了 PIN2 中的一个新磷酸化位点，并提供了证据表明它在硝酸盐对主根和侧根生长的响应中起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59f/8447600/343e0afbfcef/EMBR-22-e51813-g001.jpg
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