Gaskell S J, Rollins K, Smith R W, Parker C E
Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath, UK.
Biomed Environ Mass Spectrom. 1987 Dec;14(12):717-22. doi: 10.1002/bms.1200141205.
The determination of serum cortisol by thermospray liquid chromatography/mass spectrometry (LC/MS) has been assessed. The method incorporates stable isotope dilution and immunoadsorption extraction. [M + H]+ ions are monitored during LC/MS. The within-assay reproducibility is satisfactory (coefficient of variation 7% at a concentration of 190 ng ml-1), though inferior to that achieved with the (more lengthy) procedure of gas chromatography/mass spectrometry (GC/MS). Satisfactory agreement between LC/MS (y) and GC/MS (x) data was observed (y = 0.934x + 12.4 ng ml-1; r = 0.968; n = 14). It is concluded that the generation of precise reference data for the assessment of routine cortisol assays is at present better achieved by GC/MS. LC/MS, however, provides satisfactory quantitative data via a more simple and rapid method.
已对采用热喷雾液相色谱/质谱联用（LC/MS）法测定血清皮质醇进行了评估。该方法采用稳定同位素稀释和免疫吸附萃取。在LC/MS过程中监测[M + H]+离子。批内重复性令人满意（在浓度为190 ng/ml时变异系数为7%），尽管不如气相色谱/质谱联用（GC/MS）（更为繁琐）的方法所达到的重复性。观察到LC/MS（y）和GC/MS（x）数据之间具有良好的一致性（y = 0.934x + 12.4 ng/ml；r = 0.968；n = 14）。得出的结论是，目前通过GC/MS能更好地生成用于评估常规皮质醇测定的精确参考数据。然而，LC/MS通过一种更简单快速的方法提供了令人满意的定量数据。
