Thornton S N, Sirinathsinghji D J, Delaney C E
A.F.R.C., Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Brain Res. 1987 Dec 29;437(2):339-44. doi: 10.1016/0006-8993(87)91648-9.
Colchicine was injected into the region of the organum vasculosum lamina terminalis/anterior region of the third cerebral ventricle (OVLT/AV3V) to produce a temporary disruption of the nervous connections of this area and the rest of the anterior hypothalamus, but to maintain the vascular connections intact. Rats were kept in metabolism cages throughout the experiment and food and water intake plus urine and electrolyte excretion and body weight were measured each day. Food intake, body weight gain and urine and sodium excretion were reduced for several days after the injection of colchicine and the rats went into a marked positive sodium balance from the third day postinjection. Following 24 h water deprivation, 7 days after the colchicine injection, water intake was increased for 2 days. Urine and electrolyte excretion and food intake were also increased on the second day after the deprivation. Following a second deprivation, 10 days later, the colchicine-injected animals behaved as the control rats had done during both the deprivation periods. Injections of colchicine into the OVLT region of the AV3V, that would have blocked neural activity while maintaining a constant blood supply, produced some of the characteristics of a 'normal' lesion in this area; the rats decreased sodium excretion and increased their water intake in response to water deprivation. Therefore, colchicine may provide a useful means of investigating what role the constituent areas of the AV3V play in body fluid regulation.
将秋水仙碱注射到终板血管器/第三脑室前区（OVLT/AV3V）区域，以暂时破坏该区域与下丘脑前部其他区域的神经连接，但保持血管连接完整。在整个实验过程中，将大鼠饲养在代谢笼中，每天测量食物和水的摄入量、尿液和电解质排泄量以及体重。注射秋水仙碱后的几天内，食物摄入量、体重增加以及尿液和钠排泄量均减少，并且从注射后的第三天开始，大鼠进入明显的正钠平衡状态。在注射秋水仙碱7天后，经过24小时禁水，大鼠的饮水量增加了2天。禁水后的第二天，尿液和电解质排泄量以及食物摄入量也增加了。10天后进行第二次禁水时，注射秋水仙碱的动物在两个禁水期的表现与对照大鼠相同。向AV3V的OVLT区域注射秋水仙碱，在保持恒定血液供应的同时阻断神经活动，产生了该区域“正常”损伤的一些特征；大鼠在禁水时减少钠排泄并增加饮水量。因此，秋水仙碱可能为研究AV3V的组成区域在体液调节中所起的作用提供一种有用的方法。
