Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas e da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil; Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
Acta Trop. 2021 Nov;223:106074. doi: 10.1016/j.actatropica.2021.106074. Epub 2021 Aug 4.
Free living amoebae (FLA) can be found in different environments, where they feed on diverse microorganisms. Some bacteria preyed by FLA are called amoeba-resistant bacteria (ARB), as they can resist to lysosomal fusion and are capable of multiplying and evading FLA after internalization, propagating in the environment. Despite the health risks due to the existence of pathogenic and opportunistic species that are ARB and the pathogenicity of some FLA species, there are no water quality protocols to analyze the presence of ARB or FLA. In this sense, our study aimed to isolate FLA through amoebal enrichment and to identify ARB using amoebal coculture in water samples from a public park and two hospitals in southern Brazil. As a result, 9 different microorganisms genera have been identified through amoebal coculture, including fastidious Legionella spp. and Bosea vestrisii. From the positive samples for FLA, by amoebal enrichment, Acanthamoeba spp., Vermamoeba vermiformis and Naegleria spp. were identified in 14 amoebic isolates. The methodologies used in this work proved to be effective as simple and low-cost methods to be used in the implementation in water quality control of anthropogenic environments.
自由生活阿米巴（FLA）可以在不同的环境中找到，它们以各种微生物为食。一些被 FLA 捕食的细菌被称为阿米巴抗性细菌（ARB），因为它们可以抵抗溶酶体融合，并在被内化后能够增殖和逃避 FLA，在环境中传播。尽管存在致病性和机会性物种以及某些 FLA 物种的致病性，导致健康风险，但没有水质协议来分析 ARB 或 FLA 的存在。在这种情况下，我们的研究旨在通过阿米巴富集分离 FLA，并在巴西南部的一个公园和两家医院的水样中通过阿米巴共培养来鉴定 ARB。结果，通过阿米巴共培养鉴定出了 9 个不同的微生物属，包括难以培养的军团菌属和 Bosea vestrisii。从 FLA 的阳性样本中，通过阿米巴富集，在 14 个阿米巴分离物中鉴定出了棘阿米巴属、变形虫属和内格里亚属。本工作中使用的方法被证明是有效的，因为它们是简单和低成本的方法，可用于人为环境的水质控制。
