Gong Yi, Chen Jiao, Cui Yalei, Li Shengkang
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Institute of Marine Sciences, Shantou University, Shantou, 515063, China.
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China.
Dev Comp Immunol. 2021 Dec;125:104217. doi: 10.1016/j.dci.2021.104217. Epub 2021 Aug 3.
Caspase 2 is widely studied for its function in the regulation of apoptosis in mammals. Despite the fundamental role of apoptosis during the anti-viral immune response, the relationship between Caspase 2 and virus infection has not been extensively explored in invertebrates. Also, whether or not miRNAs involve this process remains unclear. To address this issue, the miRNA-mediated regulation of Caspase 2 in mud crab (Scylla paramamosain) (Sp-Caspase 2) was characterized in this study. Sp-Caspase 2 contains an open reading frame (ORF) of 969 bp encoding 322 deduced amino acids and possesses a conserved CASc domain. The results suggested that Sp-Caspase 2 could suppress white spot syndrome virus infection via apoptosis induction. The further data showed that Sp-Caspase 2 was directly targeted by miR-2 in mud crab. Silencing or overexpression of miR-2 could affect apoptosis and WSSV replication through the regulation of Sp-Caspase 2 expression. Taken together, these results demonstrated the crucial role of the miR-2-Caspase 2 pathway in the innate immunity of mud crabs and revealed a novel mechanism in the anti-viral immune response in marine invertebrates.
半胱天冬酶2因其在哺乳动物细胞凋亡调控中的作用而被广泛研究。尽管细胞凋亡在抗病毒免疫反应中具有重要作用，但半胱天冬酶2与病毒感染之间的关系在无脊椎动物中尚未得到广泛探索。此外，miRNA是否参与这一过程仍不清楚。为了解决这个问题，本研究对中华绒螯蟹（Scylla paramamosain）（Sp-Caspase 2）中miRNA介导的半胱天冬酶2调控进行了表征。Sp-Caspase 2包含一个969 bp的开放阅读框（ORF），编码322个推导氨基酸，并具有一个保守的CASc结构域。结果表明，Sp-Caspase 2可通过诱导细胞凋亡抑制白斑综合征病毒感染。进一步的数据表明，Sp-Caspase 2是中华绒螯蟹中miR-2的直接靶点。miR-2的沉默或过表达可通过调节Sp-Caspase 2的表达影响细胞凋亡和WSSV复制。综上所述，这些结果证明了miR-2-Caspase 2途径在中华绒螯蟹先天免疫中的关键作用，并揭示了海洋无脊椎动物抗病毒免疫反应中的一种新机制。
