Schmid Christian, Sharma Sapna, Stark Timo D, Günzkofer Daniela, Hofmann Thomas F, Ulrich Detlef, Dunemann Frank, Nothnagel Thomas, Dawid Corinna
Food Chemistry and Molecular Sensory Science, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Königin-Luise-Straße 19, 14195 Berlin, Germany.
Foods. 2021 Jul 11;10(7):1607. doi: 10.3390/foods10071607.
Cultivated carrot is one of the most important vegetable plants in the world and favored by consumers for its typically sweet flavor. Unfortunately, the attractive sensory quality is hindered by a sporadic bitter off-taste. To evaluate the influence of the abiotic stress conditions, waterlogging and drought, on the bitter sensometabolome as well as agronomical traits of six genotypes of , a field trial was performed. Enabling the accurate tracing of carrots' bitter compounds and therefore their metabolic changes, a fast and robust high-throughput UHPLC-MS/MS was developed and validated. Remarkably, the genotypes are the driving source for the biological fate of the bitter metabolites that are reflected in concentrations, dose-over-threshold factors, and fold changes. A certain influence of the irrigation level is observable but is overruled by its cultivar. Therefore, metabolic stress response in carrots seems to be genotype dependent. Hence, this study might help to plant specific carrot genotypes that are adapted to stress conditions evoked by future climatic changes.
栽培胡萝卜是世界上最重要的蔬菜作物之一，因其典型的甜味而受到消费者喜爱。不幸的是，这种诱人的感官品质受到偶尔出现的苦味异味的影响。为了评估非生物胁迫条件（涝害和干旱）对六种基因型胡萝卜苦味感官代谢组以及农艺性状的影响，进行了一项田间试验。为了能够准确追踪胡萝卜的苦味化合物及其代谢变化，开发并验证了一种快速、可靠的高通量超高效液相色谱-串联质谱法（UHPLC-MS/MS）。值得注意的是，基因型是苦味代谢产物生物命运的驱动源，这体现在浓度、剂量超过阈值因子和倍数变化上。灌溉水平有一定影响，但被品种所主导。因此，胡萝卜的代谢应激反应似乎取决于基因型。因此，本研究可能有助于种植适应未来气候变化引发的胁迫条件的特定胡萝卜基因型。
