• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

弥漫性PRAME表达在区分薄型黑色素瘤与重度发育异常痣方面具有高度特异性，但无法区分转移性与非转移性薄型黑色素瘤。

Diffuse PRAME Expression Is Highly Specific for Thin Melanomas in the Distinction from Severely Dysplastic Nevi but Does Not Distinguish Metastasizing from Non-Metastasizing Thin Melanomas.

作者信息

Gassenmaier Maximilian, Hahn Matthias, Metzler Gisela, Bauer Jürgen, Yazdi Amir Sadegh, Keim Ulrike, Garbe Claus, Wagner Nikolaus Benjamin, Forchhammer Stephan

机构信息

Department of Dermatology, Eberhard Karls University of Tübingen, 72076 Tübingen, Germany.

Dermatologie in Stuttgart, 70173 Stuttgart, Germany.

出版信息

Cancers (Basel). 2021 Jul 31;13(15):3864. doi: 10.3390/cancers13153864.

DOI:10.3390/cancers13153864
PMID:34359765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8345662/
Abstract

BACKGROUND

PReferentially expressed Antigen in MElanoma (PRAME) immunohistochemistry is increasingly used as diagnostic adjunct in the evaluation of melanocytic tumors. The expression and prognostic significance of PRAME in melanomas ≤1.0 mm and its diagnostic utility in the distinction from severely dysplastic compound nevi (SDN) have not been studied.

METHODS

We investigated and compared the immunohistochemical PRAME expression in 70 matched thin metastasizing and non-metastasizing melanomas and 45 nevi from patients with long-term follow-up (35 SDN and 10 unequivocally benign compound nevi).

RESULTS

Diffuse PRAME staining in >75% of lesional epidermal and dermal melanocytes identified 58.6% of thin melanomas but did not distinguish metastasizing from non-metastasizing melanomas ( = 0.81). A superficial atypical melanocytic proliferation of uncertain significance, in which the final diagnostic interpretation favored a SDN was the only nevus with diffuse PRAME expression (1/45). Melanomas and SDN with PRAME immunoreactivity exhibited different staining patterns. Most melanomas (67.6%) showed uniform PRAME expression in the in situ and invasive component, whereas most SDN (81.0%) showed a decreasing gradient with depth.

CONCLUSION

Diffuse intraepidermal and dermal PRAME staining is highly specific for melanomas in the distinction from SDN. PRAME expression is not a prognostic biomarker in melanomas ≤1.0 mm.

摘要

背景

黑色素瘤优先表达抗原（PRAME）免疫组化在黑素细胞肿瘤评估中越来越多地用作诊断辅助手段。PRAME在厚度≤1.0 mm的黑色素瘤中的表达及预后意义，以及其在与重度发育异常复合痣（SDN）鉴别诊断中的诊断效用尚未得到研究。

方法

我们调查并比较了70例配对的薄型转移性和非转移性黑色素瘤以及45例长期随访患者的痣（35例SDN和10例明确为良性的复合痣）的PRAME免疫组化表达情况。

结果

病变表皮和真皮黑素细胞中>75%出现弥漫性PRAME染色可识别58.6%的薄型黑色素瘤，但无法区分转移性和非转移性黑色素瘤（P = 0.81）。唯一出现弥漫性PRAME表达的痣是最终诊断倾向于SDN的意义不确定的浅表非典型黑素细胞增殖（1/45）。具有PRAME免疫反应性的黑色素瘤和SDN表现出不同的染色模式。大多数黑色素瘤（67.6%）在原位和浸润成分中表现出均匀的PRAME表达，而大多数SDN（81.0%）则表现出随深度递减的梯度。

结论

表皮内和真皮内弥漫性PRAME染色在与SDN鉴别时对黑色素瘤具有高度特异性。PRAME表达不是厚度≤1.0 mm黑色素瘤的预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/92bd404e911a/cancers-13-03864-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/743dc56262f5/cancers-13-03864-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/afe75fa8152a/cancers-13-03864-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/e1e6d17ebc8c/cancers-13-03864-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/584a6f7e2b6e/cancers-13-03864-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/cf544d2fca44/cancers-13-03864-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/92bd404e911a/cancers-13-03864-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/743dc56262f5/cancers-13-03864-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/afe75fa8152a/cancers-13-03864-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/e1e6d17ebc8c/cancers-13-03864-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/584a6f7e2b6e/cancers-13-03864-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/cf544d2fca44/cancers-13-03864-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9c/8345662/92bd404e911a/cancers-13-03864-g007.jpg

相似文献

1
Diffuse PRAME Expression Is Highly Specific for Thin Melanomas in the Distinction from Severely Dysplastic Nevi but Does Not Distinguish Metastasizing from Non-Metastasizing Thin Melanomas.弥漫性PRAME表达在区分薄型黑色素瘤与重度发育异常痣方面具有高度特异性，但无法区分转移性与非转移性薄型黑色素瘤。
Cancers (Basel). 2021 Jul 31;13(15):3864. doi: 10.3390/cancers13153864.
2
PRAME Immunohistochemistry in Thin Melanomas Compared to Melanocytic Nevi.与黑素细胞痣相比，薄型黑色素瘤中PRAME的免疫组织化学研究
Diagnostics (Basel). 2024 Sep 12;14(18):2015. doi: 10.3390/diagnostics14182015.
3
PRAME Expression in Melanocytic Tumors.黑色素细胞肿瘤中的 PRAME 表达。
Am J Surg Pathol. 2018 Nov;42(11):1456-1465. doi: 10.1097/PAS.0000000000001134.
4
Preferentially Expressed Antigen in Melanoma Immunostaining in a Series of Melanocytic Neoplasms.一系列黑素细胞肿瘤中黑色素瘤免疫染色中优先表达的抗原
Am J Dermatopathol. 2021 Nov 1;43(11):794-800. doi: 10.1097/DAD.0000000000001885.
5
PRAME expression in melanocytic proliferations with intermediate histopathologic or spitzoid features.具有中间组织病理学或 Spitz 样特征的黑色素细胞增生中 PRAME 的表达。
J Cutan Pathol. 2020 Dec;47(12):1123-1131. doi: 10.1111/cup.13818. Epub 2020 Sep 10.
6
Diagnostic utility of combining PRAME and HMB-45 stains in primary melanocytic tumors.联合应用 PRAME 和 HMB-45 染色在原发性黑色素细胞瘤中的诊断效用。
Ann Diagn Pathol. 2023 Dec;67:152211. doi: 10.1016/j.anndiagpath.2023.152211. Epub 2023 Sep 13.
7
Potential diagnostic utility of PRAME and p16 immunohistochemistry in melanocytic nevi and malignant melanoma.PRAME 和 p16 免疫组化在黑素细胞痣和恶性黑色素瘤中的潜在诊断效用。
J Cutan Pathol. 2023 Aug;50(8):763-772. doi: 10.1111/cup.14438. Epub 2023 Apr 27.
8
PRAME expression by immunohistochemistry and reverse transcription quantitative PCR in conjunctival melanocytic lesions-a comprehensive clinicopathologic study of 202 cases and correlation of cytogenetics with PRAME expression in challenging conjunctival melanocytic lesions.通过免疫组织化学和逆转录定量PCR检测PRAME在结膜黑素细胞性病变中的表达——202例综合临床病理研究以及在具有挑战性的结膜黑素细胞性病变中细胞遗传学与PRAME表达的相关性
Hum Pathol. 2023 Apr;134:1-18. doi: 10.1016/j.humpath.2023.02.002. Epub 2023 Feb 16.
9
Refining the application of PRAME-a useful marker in high CSD and acral melanoma subtypes.细化 PRAME 标志物的应用——在高 CSD 和肢端黑色素瘤亚型中具有重要价值。
Virchows Arch. 2023 Dec;483(6):847-854. doi: 10.1007/s00428-023-03648-w. Epub 2023 Sep 19.
10
Preferentially expressed antigen in melanoma and p16 expression in acral melanocytic neoplasms.黑色素瘤中优先表达的抗原和肢端黑素细胞性肿瘤中的 p16 表达。
J Cutan Pathol. 2022 Mar;49(3):220-230. doi: 10.1111/cup.14130. Epub 2021 Sep 20.

引用本文的文献

1
Therapeutic Opportunities in Melanoma Through PRAME Expression.通过黑色素瘤抗原基因（PRAME）表达实现黑色素瘤的治疗机会
Biomedicines. 2025 Aug 15;13(8):1988. doi: 10.3390/biomedicines13081988.
2
Enhanced diagnostic potential of CSPG4 in melanoma and nevi: a comparative study with PRAME, CDC7 and Ki67.CSPG4在黑色素瘤和痣中的诊断潜力增强：与PRAME、CDC7和Ki67的比较研究
J Pathol. 2025 Jul 23. doi: 10.1002/path.6450.
3
Deep learning reconstruction for accelerated 3-D magnetic resonance cholangiopancreatography.用于加速三维磁共振胰胆管造影的深度学习重建

本文引用的文献

1
The utility of PRAME staining in identifying malignant transformation of melanocytic nevi.PRAME 染色在鉴定黑色素痣恶性转化中的效用。
J Cutan Pathol. 2021 Jul;48(7):856-862. doi: 10.1111/cup.13958. Epub 2021 Feb 3.
2
The Rapid Rise in Cutaneous Melanoma Diagnoses.皮肤黑色素瘤诊断数量的迅速上升。
N Engl J Med. 2021 Jan 7;384(1):72-79. doi: 10.1056/NEJMsb2019760.
3
PRAME immunohistochemistry as an adjunct for diagnosis and histological margin assessment in lentigo maligna.PRAME 免疫组化在诊断和评估恶性雀斑样痣的组织学边界中的辅助作用。
Radiol Med. 2025 Mar 18. doi: 10.1007/s11547-025-01987-z.
4
The Utilization of PRAME in the Diagnosis, Prognosis, and Treatment of Melanoma.PRAME 在黑色素瘤的诊断、预后和治疗中的应用。
Cells. 2024 Oct 20;13(20):1740. doi: 10.3390/cells13201740.
5
PRAME Immunohistochemistry in Thin Melanomas Compared to Melanocytic Nevi.与黑素细胞痣相比，薄型黑色素瘤中PRAME的免疫组织化学研究
Diagnostics (Basel). 2024 Sep 12;14(18):2015. doi: 10.3390/diagnostics14182015.
6
Appropriate Statistical Methods to Assess Cross-study Diagnostic 23-Gene Expression Profile Test Performance for Cutaneous Melanocytic Neoplasms.评估皮肤黑素细胞肿瘤跨研究诊断 23 基因表达谱检测性能的适当统计方法。
Am J Dermatopathol. 2024 Dec 1;46(12):833-838. doi: 10.1097/DAD.0000000000002808. Epub 2024 Aug 14.
7
PRAME Is an Effective Tool for the Diagnosis of Nevus-Associated Cutaneous Melanoma.PRAME是诊断痣相关皮肤黑色素瘤的有效工具。
Cancers (Basel). 2024 Jan 9;16(2):278. doi: 10.3390/cancers16020278.
8
Shedding light on PRAME expression in dysplastic nevi: a cohort study.揭示发育不良性痣中 PRAME 表达：一项队列研究。
Virchows Arch. 2024 Jul;485(1):97-104. doi: 10.1007/s00428-023-03720-5. Epub 2023 Dec 19.
9
Cutaneous Melanoma: A Review of Multifactorial Pathogenesis, Immunohistochemistry, and Emerging Biomarkers for Early Detection and Management.皮肤黑色素瘤：多因素发病机制、免疫组织化学及早期检测和管理新兴生物标志物的综述。
Int J Mol Sci. 2023 Nov 1;24(21):15881. doi: 10.3390/ijms242115881.
10
Immunohistochemistry for PRAME in Dermatopathology.免疫组织化学染色在皮肤病理中的应用。
Am J Dermatopathol. 2023 Nov 1;45(11):733-747. doi: 10.1097/DAD.0000000000002440.
Histopathology. 2021 Jun;78(7):1000-1008. doi: 10.1111/his.14312. Epub 2021 Apr 2.
4
PRAME expression in 155 cases of metastatic melanoma.PRAME 在 155 例转移性黑色素瘤中的表达。
J Cutan Pathol. 2021 Apr;48(4):479-485. doi: 10.1111/cup.13876. Epub 2020 Nov 8.
5
Differential Expression of Cancer Testis Antigens on Lentigo Maligna and Lentigo Maligna Melanoma.恶性雀斑样痣和恶性雀斑样黑素瘤中癌睾丸抗原的差异表达。
Am J Dermatopathol. 2020 Aug;42(8):625-627. doi: 10.1097/DAD.0000000000001607.
6
PRAME expression in melanocytic proliferations with intermediate histopathologic or spitzoid features.具有中间组织病理学或 Spitz 样特征的黑色素细胞增生中 PRAME 的表达。
J Cutan Pathol. 2020 Dec;47(12):1123-1131. doi: 10.1111/cup.13818. Epub 2020 Sep 10.
7
Comparison of Immunohistochemistry for PRAME With Cytogenetic Test Results in the Evaluation of Challenging Melanocytic Tumors.免疫组织化学检测 PRAME 与细胞遗传学检测结果在评估具有挑战性的黑色素细胞肿瘤中的比较。
Am J Surg Pathol. 2020 Jul;44(7):893-900. doi: 10.1097/PAS.0000000000001492.
8
Immunohistochemistry for PRAME in the Distinction of Nodal Nevi From Metastatic Melanoma.PRAME 免疫组化在结内痣与转移性黑色素瘤鉴别诊断中的作用。
Am J Surg Pathol. 2020 Apr;44(4):503-508. doi: 10.1097/PAS.0000000000001393.
9
PRAME Expression in Melanocytic Tumors.黑色素细胞肿瘤中的 PRAME 表达。
Am J Surg Pathol. 2018 Nov;42(11):1456-1465. doi: 10.1097/PAS.0000000000001134.
10
Pathologists' diagnosis of invasive melanoma and melanocytic proliferations: observer accuracy and reproducibility study.病理学家对侵袭性黑色素瘤和黑素细胞增殖的诊断：观察者准确性和可重复性研究。
BMJ. 2017 Jun 28;357:j2813. doi: 10.1136/bmj.j2813.