Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Natural Resources Institute, University of Greenwich, Chatham, Kent ME4 4TB, UK.
Cells. 2021 Jul 5;10(7):1700. doi: 10.3390/cells10071700.
Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in whole body was significantly reduced. BtVAMP2 was found to be conserved among the species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly.
双生病毒导致农业生产的巨大损失，特别是在热带和亚热带地区，它们只能由粉虱种复合体的成员传播。然而，双生病毒由其粉虱介体传播的分子机制尚不清楚。在这项研究中，我们发现囊泡相关膜蛋白 2（BtVAMP2）与番茄黄曲叶病毒（TYLCV）的外壳蛋白（CP）相互作用，TYLCV 是一种新兴的双生病毒，它严重影响着全球番茄的生产。感染 TYLCV 后，的转录增加。当通过特异性 BtVAMP2 抗体喂食来阻断 BtVAMP2 蛋白时，TYLCV 在整个 体内的数量明显减少。BtVAMP2 在粉虱种复合体中是保守的，也与斯里兰卡木薯花叶病毒（SLCMV）的 CP 相互作用。当用 BtVAMP2 抗体喂食时，SLCMV 在白 整体中的获取量也显著减少。总的来说，我们的结果表明 BtVAMP2 与双生病毒的 CP 相互作用，并促进了它们被粉虱的获取。
