Bousquet J, Lebel B, Dhivert H, Bataille Y, Martinot B, Michel F B
Clinique des Maladies Respiratoires, Hôpital l'Aiguelonque, Montpellier, France.
Clin Allergy. 1987 Nov;17(6):529-36. doi: 10.1111/j.1365-2222.1987.tb02049.x.
Nasal challenges with pollen grains are as close as possible to natural pollen exposure, but they are not well documented in grass pollen allergy. Forty-four grass pollen allergic patients and ten non-allergic volunteers were tested by means of nasal challenge, quantitative skin-prick tests with a standardized orchard grass pollen extract and serum-specific IgE. Nasal challenges were performed with lactose and increasing concentrations of orchard grass pollen grains (15-3645 grains, three-fold increase). The test was considered to be positive when a symptom score over 5 was obtained, since this score had been previously correlated with the release of PGD2 in nasal secretions. All control subjects and 3/44 patients had a negative challenge. The number of orchard pollen grains required to elicit a positive challenge was 332 +/- 440 (range: 15-1215 grains) and the distribution was Gaussian. This number is higher than expected according to pollen calendars performed during the season, but owing to the priming effect of the nasal mucosa by allergens it is compatible to natural exposure. The correlation between nasal provocation tests and skin-prick test end-points was significant (P less than 0.005, Spearman rank test). Conversely there was no correlation between nasal challenge or skin-prick test end-point and serum-specific IgE.
用花粉颗粒进行鼻腔激发试验尽可能接近自然花粉暴露情况，但在草花粉过敏方面相关记录并不完善。对44名草花粉过敏患者和10名非过敏志愿者进行了鼻腔激发试验、用标准化果园草花粉提取物进行的定量皮肤点刺试验以及血清特异性IgE检测。鼻腔激发试验使用乳糖和浓度递增的果园草花粉颗粒（15 - 3645颗粒，呈三倍递增）。当症状评分超过5分时，试验被认为呈阳性，因为该评分此前已与鼻分泌物中前列腺素D2的释放相关联。所有对照受试者和44名患者中的3名激发试验结果为阴性。引发阳性激发试验所需的果园花粉颗粒数量为332 ± 440（范围：15 - 1215颗粒），且分布呈高斯分布。根据该季节进行的花粉监测记录，这个数量高于预期，但由于变应原对鼻黏膜的启动作用，它与自然暴露情况相符。鼻腔激发试验与皮肤点刺试验终点之间的相关性显著（P < 0.005，Spearman秩和检验）。相反，鼻腔激发试验或皮肤点刺试验终点与血清特异性IgE之间无相关性。
