Department of Prosthodontics and Oral Implantology, School of Dentistry, Iwate Medical University, 1-3-27 Chuo-dori, Morioka 020-8505, Iwate, Japan.
Division of Molecular Microbiology, Department of Microbiology, Iwate Medical University, 1-1-1 Idai-dori, Morioka 028-3694, Iwate, Japan.
Int J Mol Sci. 2021 Jul 28;22(15):8084. doi: 10.3390/ijms22158084.
Titanium is often used in the medical field and in dental implants due to its biocompatibility, but it has a high rate of leading to peri-implantitis, which progresses faster than periodontitis. Therefore, in the present study, the expression of cytokines from gingival epithelial cells by nanotitania was investigated, which is derived from titanium in the oral cavity, and the additional effect of (periodontopathic bacteria) lipopolysaccharide (PgLPS) was investigated. Ca9-22 cells were used as a gingival epithelial cell model and were cultured with nanotitania alone or with PgLPS. Cytokine expression was examined by reverse transcription-quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. In addition, cellular uptake of nanotitania was observed in scanning electron microscopy images. The expression of interleukin (IL)-6 and IL-8 significantly increased in Ca9-22 cells by nanotitania treatment alone, and the expression was further increased by the presence of PgLPS. Nanotitania was observed to phagocytose Ca9-22 cells in a dose- and time-dependent manner. Furthermore, when the expression of IL-11, related to bone resorption, was investigated, a significant increase was confirmed by stimulation with nanotitania alone. Therefore, nanotitania could be associated with the onset and exacerbation of peri-implantitis, and the presence of periodontal pathogens may worsen the condition. Further clinical reports are needed to confirm these preliminary results.
钛常被应用于医学领域和牙科植入物中，因其具有生物相容性，但它会导致较高的种植体周围炎发生率，且其进展速度快于牙周炎。因此，本研究调查了源自口腔内钛的纳米二氧化钛对牙龈上皮细胞细胞因子的表达的影响，并进一步研究了（牙周病相关细菌）脂多糖（PgLPS）的附加作用。使用 Ca9-22 细胞作为牙龈上皮细胞模型，用纳米二氧化钛单独或与 PgLPS 一起培养。通过逆转录定量聚合酶链反应和酶联免疫吸附试验检查细胞因子的表达。此外，通过扫描电子显微镜图像观察纳米二氧化钛的细胞摄取。纳米二氧化钛处理可单独显著增加 Ca9-22 细胞中白细胞介素（IL）-6 和 IL-8 的表达，且存在 PgLPS 时表达进一步增加。纳米二氧化钛呈剂量和时间依赖性地吞噬 Ca9-22 细胞。此外，当研究与骨吸收相关的 IL-11 的表达时，单独用纳米二氧化钛刺激可确认其显著增加。因此，纳米二氧化钛可能与种植体周围炎的发生和恶化有关，牙周病病原体的存在可能会使病情恶化。需要进一步的临床报告来证实这些初步结果。
