Bressan Eriberto, Ferroni Letizia, Gardin Chiara, Bellin Gloria, Sbricoli Luca, Sivolella Stefano, Brunello Giulia, Schwartz-Arad Devorah, Mijiritsky Eitan, Penarrocha Miguel, Penarrocha David, Taccioli Cristian, Tatullo Marco, Piattelli Adriano, Zavan Barbara
Department of Neurosciences, University of Padova, 35133 Padova, Italy.
Maria Cecilia Hospital, GVM Care & Research, 48033 Cotignola (RA), Italy.
Materials (Basel). 2019 Jun 25;12(12):2036. doi: 10.3390/ma12122036.
Peri-implantitis is an inflammatory disease affecting tissues surrounding dental implants. Although it represents a common complication of dental implant treatments, the underlying mechanisms have not yet been fully described. The aim of this study is to identify the role of titanium nanoparticles released form the implants on the chronic inflammation and bone lysis in the surrounding tissue. We analyzed the in vitro effect of titanium (Ti) particle exposure on mesenchymal stem cells (MSCs) and fibroblasts (FU), evaluating cell proliferation by MTT test and the generation of reactive oxygen species (ROS). Subsequently, in vivo analysis of peri-implant Ti particle distribution, histological, and molecular analyses were performed. Ti particles led to a time-dependent decrease in cell viability and increase in ROS production in both MSCs and FU. Tissue analyses revealed presence of oxidative stress, high extracellular and intracellular Ti levels and imbalanced bone turnover. High expression of ZFP467 and the presence of adipose-like tissue suggested dysregulation of the MSC population; alterations in vessel morphology were identified. The results suggest that Ti particles may induce the production of high ROS levels, recruiting abnormal quantity of neutrophils able to produce high level of metalloproteinase. This induces the degradation of collagen fibers. These events may influence MSC commitment, with an imbalance of bone regeneration.
种植体周炎是一种影响牙种植体周围组织的炎症性疾病。虽然它是牙种植治疗的常见并发症，但其潜在机制尚未完全阐明。本研究的目的是确定种植体释放的钛纳米颗粒在周围组织慢性炎症和骨溶解中的作用。我们分析了钛（Ti）颗粒暴露对间充质干细胞（MSCs）和成纤维细胞（FUs）的体外影响，通过MTT试验评估细胞增殖，并检测活性氧（ROS）的产生。随后，对种植体周围Ti颗粒分布进行了体内分析，并进行了组织学和分子分析。Ti颗粒导致MSCs和FUs的细胞活力随时间下降，ROS产生增加。组织分析显示存在氧化应激、细胞外和细胞内Ti水平升高以及骨转换失衡。ZFP467的高表达和脂肪样组织的存在提示MSCs群体失调；发现血管形态改变。结果表明，Ti颗粒可能诱导高水平ROS的产生，招募异常数量的能够产生高水平金属蛋白酶的中性粒细胞。这会导致胶原纤维降解。这些事件可能影响MSCs的分化，导致骨再生失衡。
