Adarska Petia, Wong-Dilworth Luis, Bottanelli Francesca
Institut für Biochemie, Freie Universität Berlin, Berlin, Germany.
Front Cell Dev Biol. 2021 Jul 22;9:679046. doi: 10.3389/fcell.2021.679046. eCollection 2021.
Molecular switches of the ADP-ribosylation factor (ARF) GTPase family coordinate intracellular trafficking at all sorting stations along the secretory pathway, from the ER-Golgi-intermediate compartment (ERGIC) to the plasma membrane (PM). Their GDP-GTP switch is essential to trigger numerous processes, including membrane deformation, cargo sorting and recruitment of downstream coat proteins and effectors, such as lipid modifying enzymes. While ARFs (in particular ARF1) had mainly been studied in the context of coat protein recruitment at the Golgi, COPI/clathrin-independent roles have emerged in the last decade. Here we review the roles of human ARF1-5 GTPases in cellular trafficking with a particular emphasis on their roles in post-Golgi secretory trafficking and in sorting in the endo-lysosomal system.
ADP核糖基化因子（ARF）GTP酶家族的分子开关在沿着分泌途径的所有分选站协调细胞内运输，从内质网-高尔基体中间区室（ERGIC）到质膜（PM）。它们的GDP-GTP开关对于触发众多过程至关重要，包括膜变形、货物分选以及下游衣被蛋白和效应器（如脂质修饰酶）的募集。虽然ARF（特别是ARF1）主要是在高尔基体衣被蛋白募集的背景下进行研究的，但在过去十年中出现了不依赖COPI/网格蛋白的作用。在这里，我们综述了人类ARF1-5 GTP酶在细胞运输中的作用，特别强调它们在高尔基体后分泌运输和内溶酶体系统分选中的作用。
