Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
Biomed Res Int. 2021 Jul 28;2021:2252705. doi: 10.1155/2021/2252705. eCollection 2021.
To observe the effects of luteolin on galactosamine (D-Gal)/lipopolysaccharide (LPS) induced liver injury in mice. Male C57BL/6 mice were randomly divided into 4 groups: normal control group, D-GaI/LPS group, D-GaI/LPS + luteolin (Lu, 20 mg/kg), and D-GaI/LPS + luteolin (Lu, 40 mg/kg). Mice in the normal control group and D-GaI/LPS group were given distilled water while other groups were given drugs in 7 days by gavage. 4 hours after the continuous administration, Gal (700 mg/kg) and LPS (10 mg/kg) were injected intraperitoneally. Mice in the normal control group were given the same volume of vegetable oil solution. 24 h after the establishment of the mice model, blood and liver samples were collected. Hematoxylin (HE) staining was used to observe the changes of hepatic histopathology. Alanine aminotransferase (ALT) and glutamic oxalacetic transaminase (AST) in serum, interleukin-6 (IL-6), interleukin-1 (IL-1), and tumor necrosis factor (TNF-) were measured by related kits. Western blotting was used to demonstrate the expression levels of related inflammation proteins. Lu significantly reduced levels of proinflammatory cytokines including interleukin-6 (IL-6), interleukin-1 (IL-1), and tumor necrosis factor- (TNF-) in serum and liver. Lu restored the pathological changes after galactosamine (D-Gal)/lipopolysaccharide (LPS) treatment. In addition, Lu regulated proteins levels of the NLRP3/NF-B pathway in liver. Lu exhibited therapeutical effects on D-GaI/LPS induced liver injury in mice which might be related to the regulation of the NLRP3/NF-B pathway.
观察木犀草素（Luteolin）对半乳糖胺（D-Gal）/脂多糖（LPS）诱导的小鼠肝损伤的影响。雄性 C57BL/6 小鼠随机分为 4 组：正常对照组、D-Gal/LPS 组、D-Gal/LPS+木犀草素（Lu，20mg/kg）组和 D-Gal/LPS+木犀草素（Lu，40mg/kg）组。正常对照组和 D-Gal/LPS 组给予蒸馏水，其他组连续给药 7 天，灌胃给药。连续给药 4 小时后，腹腔注射 Gal（700mg/kg）和 LPS（10mg/kg）。正常对照组给予等体积植物油溶液。造模 24h 后采集血样和肝样。苏木精（HE）染色观察肝组织病理学变化。采用相关试剂盒检测血清丙氨酸氨基转移酶（ALT）、谷草转氨酶（AST）、白细胞介素-6（IL-6）、白细胞介素-1（IL-1）和肿瘤坏死因子-（TNF-）。Western blot 法检测相关炎症蛋白表达水平。Lu 可显著降低血清和肝组织中促炎细胞因子白细胞介素-6（IL-6）、白细胞介素-1（IL-1）和肿瘤坏死因子-（TNF-）的水平。Lu 恢复了半乳糖胺（D-Gal）/脂多糖（LPS）处理后的病理变化。此外，Lu 调节了肝组织中 NLRP3/NF-B 通路相关蛋白的水平。Lu 对 D-Gal/LPS 诱导的小鼠肝损伤具有治疗作用，可能与调节 NLRP3/NF-B 通路有关。
