Rude R A, Peeler J T, Risty N G
Food and Drug Administration, Center for Microbiological Investigations, Minneapolis, MN 55401.
J Assoc Off Anal Chem. 1987 Nov-Dec;70(6):1000-2.
Ethyl acetate and diethyl ether were compared for their ability to recover Ascaris spp. and Trichuris spp. eggs from seeded milorganite, liquid sludge, and cabbage. Concentrations of 10, 100, and 1000 eggs/10 g test sample were prepared for 20 replicates of each product. The use of diethyl ether yielded fewer eggs/10 g than did ethyl acetate in 5 of 6 sets of data. For Ascaris spp., recovery from cabbage was 10 times higher with ethyl acetate at the higher concentration than with diethyl ether. For Trichuris spp., recovery from liquid sludge was slightly higher with diethyl ether for all egg concentrations. The other results ranged from 0 to 23% difference in recovery for the 2 agents. Depending on the parasites in question and the products to be screened, the substitution of ethyl acetate for diethyl ether may be significant.
比较了乙酸乙酯和乙醚从接种了蛔虫属和鞭虫属虫卵的活性污泥、液体污泥和卷心菜中回收虫卵的能力。针对每种产品制备了浓度为10、100和1000个虫卵/10克测试样品，每种浓度进行20次重复实验。在6组数据中的5组中，使用乙醚回收的虫卵/10克数量少于乙酸乙酯。对于蛔虫属，在较高浓度下，乙酸乙酯从卷心菜中回收的虫卵数量比乙醚高10倍。对于鞭虫属，在所有虫卵浓度下，乙醚从液体污泥中回收的虫卵数量略高于乙酸乙酯。其他结果显示，两种试剂的回收差异在0%至23%之间。根据所涉及的寄生虫和待筛查的产品，用乙酸乙酯替代乙醚可能具有重要意义。
