Institute of Engineering Medicine, Beijing Institute of Technology, Beijing, 100081, China.
Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Lasers Med Sci. 2022 Mar;37(2):1299-1309. doi: 10.1007/s10103-021-03388-w. Epub 2021 Aug 9.
To explore a 3.8-µm laser-induced damage and wound healing effect, we propose using optical coherence tomography (OCT) and a noninvasive monitoring-based in vivo evaluation method to quantitatively and qualitatively analyze the time-dependent biological effect of a 3.8-µm laser. The optical attenuation coefficient (OAC) is computed using a Fourier-domain algorithm. Three-dimensional (3-D) visualization of OCT images has been implemented to visualize the burnt spots. Furthermore, the burnt spots from the 3-D volumetric data was segmented and visualized, and the quantitative parameters of the burnt spots, such as the mean OACs, areas, and volumes, were computed. Then, OCT images and histological sections were analyzed to compare the structural changes. Within a certain radiation range, there is a linear relationship between radiation dose and temperature. Dermoscopic images, OCT images, and histological sections showed that, within a certain dose range, as the radiation doses increased, the cutaneous damage became more serious. One hour after laser radiation, the mean OACs increased and then decreased; the areas of burnt spots always increased and were 0.95 ± 0.07, 1.01 ± 0.06, 1.025 ± 0.07, 0.99 ± 0.07, 0.98 ± 0.07, 1.00 ± 0.07, 0.96 ± 0.05, and 0.98 ± 0.06 mm, respectively; the areas were 2.10 ± 0.63, 3.75 ± 1.85, 5.95 ± 1.62, 8.35 ± 0.88, 9.44 ± 1.28, 10.29 ± 0.49, 12.27 ± 0.96, and 13.127 ± 1.90 mm; and the volumes were 1.54 ± 0.41, 2.86 ± 0.09, 3.73 ± 0.49, 4.14 ± 0.80, 7.21 ± 0.52, 6.77 ± 0.45, 8.36 ± 0.25, and 10.65 ± 0.51 mm; and 21 days after laser radiation, the volumes were 0.67 ± 0.18, 1.64 ± 0.08, 1.87 ± 0.12, 2.57 ± 0.34, 3.43 ± 0.26, 3.64 ± 0.04, 3.84 ± 0.15, and 4.16 ± 0.53 mm, respectively. We investigated the time-dependent biological effect of 3.8-µm laser-induced cutaneous damage and wound healing using the quantitative parameters of OCT imaging and noninvasive monitoring. The real-time temperature reflects the photothermal effect during laser radiation of mouse skin. OCT images of burnt spots were segmented to compute the mean OACs, burnt area, and quantitative volumes. This study has the potential for in vivo noninvasive and quantitative clinical evaluation in the future.
为了探索 3.8μm 激光诱导的损伤和愈合效应，我们提出了使用光学相干断层扫描（OCT）和基于非侵入性监测的体内评价方法，定量和定性地分析 3.8μm 激光的时间依赖性生物学效应。采用傅里叶域算法计算光学衰减系数（OAC）。对 OCT 图像进行三维（3D）可视化，以可视化烧伤斑。此外，对 3D 体积数据的烧伤斑进行分割和可视化，并计算烧伤斑的定量参数，如平均 OACs、面积和体积。然后，对 OCT 图像和组织学切片进行分析，以比较结构变化。在一定的辐射范围内，辐射剂量和温度之间存在线性关系。皮肤镜图像、OCT 图像和组织学切片显示，在一定剂量范围内，随着辐射剂量的增加，皮肤损伤变得更加严重。激光辐射后 1 小时，平均 OACs 先升高后降低；烧伤斑的面积始终增加，分别为 0.95±0.07、1.01±0.06、1.025±0.07、0.99±0.07、0.98±0.07、1.00±0.07、0.96±0.05 和 0.98±0.06mm；面积分别为 2.10±0.63、3.75±1.85、5.95±1.62、8.35±0.88、9.44±1.28、10.29±0.49、12.27±0.96 和 13.127±1.90mm；体积分别为 1.54±0.41、2.86±0.09、3.73±0.49、4.14±0.80、7.21±0.52、6.77±0.45、8.36±0.25 和 10.65±0.51mm；激光辐射后 21 天，体积分别为 0.67±0.18、1.64±0.08、1.87±0.12、2.57±0.34、3.43±0.26、3.64±0.04、3.84±0.15 和 4.16±0.53mm。我们使用 OCT 成像和非侵入性监测的定量参数研究了 3.8μm 激光诱导皮肤损伤和愈合的时间依赖性生物学效应。实时温度反映了激光辐射小鼠皮肤期间的光热效应。对烧伤斑的 OCT 图像进行分割，以计算平均 OACs、烧伤面积和定量体积。这项研究为未来的体内非侵入性和定量临床评估提供了可能性。
