Chou H J, Yates R L, Wenninger J A
Food and Drug Administration, Division of Colors and Cosmetics, Washington, DC 20204.
J Assoc Off Anal Chem. 1987 Nov-Dec;70(6):960-3.
Cosmetic products were screened for total N-nitroso compounds by chemiluminescent measurement of nitric oxide liberated by the reductive cleavage of the N-nitroso group. The cosmetic was first partitioned between methylene chloride and water to separate polar and nonpolar N-nitroso compounds. Each extract was then examined for the presence of N-nitroso compounds by adding the cleavage reagent and sweeping the nitric oxide formed into a chemiluminescent analyzer. Although the method is not intended to be quantitative, recovery studies were conducted to determine measurable levels. Recovery studies of polar N-nitroso compounds were conducted by adding N-nitrosodiethanolamine (NDELA) to a cream, a shampoo, and a lotion at 3 levels, i.e., 80, 320, and 960 ppb, and then determining NDELA by the method. Recoveries ranged from 48 to 83% (mean 68%; SD = 11.9). For recoveries of nonpolar N-nitroso compounds, 100, 200, and 500 ppb of N-nitrosomethyltetradecylamine were added to the 3 cosmetic products. Recoveries ranged from 58 to 70% (mean 63%; SD = 5.3).
通过对N-亚硝基基团还原裂解所释放的一氧化氮进行化学发光测量，对化妆品中的总N-亚硝基化合物进行筛选。首先将化妆品在二氯甲烷和水之间进行分配，以分离极性和非极性N-亚硝基化合物。然后，通过加入裂解试剂并将生成的一氧化氮吹扫到化学发光分析仪中，对每种提取物进行N-亚硝基化合物的检测。尽管该方法并非用于定量分析，但仍进行了回收率研究以确定可测量水平。通过向面霜、洗发水和乳液中分别添加3种浓度水平（即80、320和960 ppb）的N-亚硝基二乙醇胺（NDELA），然后用该方法测定NDELA，进行了极性N-亚硝基化合物的回收率研究。回收率范围为48%至83%（平均68%；标准差 = 11.9）。对于非极性N-亚硝基化合物的回收率，向3种化妆品中添加了100、200和500 ppb的N-亚硝基甲基十四烷基胺。回收率范围为58%至70%（平均63%；标准差 = 5.3）。
