Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.
Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.
Am J Trop Med Hyg. 2021 Aug 9;105(5):1193-1197. doi: 10.4269/ajtmh.21-0479.
The recent introduction of large-scale, population-based serologic surveys in several nations where human African trypanosomiasis (HAT) remains endemic could provide an opportunity to better map the remaining disease foci and to identify asymptomatic, seropositive individuals who are infected with the more chronic form of the parasite, Trypanosoma brucei gambiense (gHAT). We have incorporated a soluble form of variant surface glycoprotein 117 and a recombinant invariant surface glycoprotein 65.1 into a multiplex bead assay (MBA) method that is commonly used for the detection of IgG antibody responses to other neglected tropical diseases. A positive result was defined as reactivity to both antigens. MBA sensitivity and specificity for gHAT infection were 92% and 96%, respectively. Assay specificity for the acute form of disease caused by T.b. rhodesiense (rHAT) was 94%, but the sensitivity was only 63.6%. In the future, additional antigens could be incorporated into the multiplex assay to improve rHAT sensitivity.
最近在一些仍存在人类非洲锥虫病（HAT）地方性流行的国家开展了大规模的基于人群的血清学调查，这为更好地绘制剩余的疾病焦点地图以及识别感染更慢性寄生虫——冈比亚锥虫（gHAT）的无症状、血清阳性个体提供了机会。我们将一种可溶性变体表面糖蛋白 117 和一种重组不变表面糖蛋白 65.1 纳入了一种多重珠粒分析（MBA）方法中，该方法常用于检测针对其他被忽视的热带病的 IgG 抗体反应。阳性结果定义为对两种抗原均有反应。该 MBA 方法对 gHAT 感染的灵敏度和特异性分别为 92%和 96%。该检测方法对由 T.b. rhodesiense（rHAT）引起的急性疾病的特异性为 94%，但灵敏度仅为 63.6%。未来，可以将其他抗原纳入多重检测方法，以提高 rHAT 的灵敏度。
