Sánchez Luis-Felipe, Cánepa Jimmy, Kim Suyeon, Nakamatsu Javier
Science Department, Pontificia Universidad Catolica del Peru-PUCP, Av. Universitaria 1801, Lima 32, Peru.
Engineering Department, Pontificia Universidad Catolica del Peru-PUCP, Av. Universitaria 1801, Lima 32, Peru.
Polymers (Basel). 2021 Jul 22;13(15):2415. doi: 10.3390/polym13152415.
Chitin is a structural polysaccharide that is found in crustaceans, insects, fungi and some yeasts. Chitin deacetylation produces chitosan, a well-studied biopolymer with reported chemical and biological properties for diverse potential applications for drug delivery, metal ion absorption, scaffolds and tissue engineering. Most known properties of chitosan have been determined from samples obtained from a variety of sources and in different conditions, this is, from chitosans with a wide range of degrees of N-acetylation (DA) and molecular weight (MW). However, as for any copolymer, the physicochemical and mechanical characteristics of chitosan highly depend on their monomer composition (DA) and chain size (MW). This work presents a simple methodology to produce chitosans with specific and predictive DA and MW. Reaction with acetic anhydride proved to be an efficient method to control the acetylation of chitosan, DAs between 10.6% and 50.6% were reproducibly obtained. In addition to this, MWs of chitosan chains were reduced in a controlled manner in two ways, by ultrasound and by acidic hydrolysis at different temperatures, samples with MWs between 130 kDa and 1300 kDa were obtained. DAs were determined by H-NMR and MWs by gel permeation chromatography.
几丁质是一种结构多糖，存在于甲壳类动物、昆虫、真菌和一些酵母中。几丁质脱乙酰作用产生壳聚糖，这是一种经过充分研究的生物聚合物，据报道其具有化学和生物学特性，在药物递送、金属离子吸收、支架和组织工程等多种潜在应用中具有价值。壳聚糖的大多数已知特性是从各种来源和不同条件下获得的样品中确定的，也就是说，来自具有广泛N-乙酰化度（DA）和分子量（MW）的壳聚糖。然而，对于任何共聚物来说，壳聚糖的物理化学和机械特性高度依赖于其单体组成（DA）和链尺寸（MW）。这项工作提出了一种简单的方法来生产具有特定和可预测的DA和MW的壳聚糖。与乙酸酐反应被证明是控制壳聚糖乙酰化的有效方法，可重复性地获得10.6%至50.6%之间的DA。除此之外，壳聚糖链的MW通过两种方式以可控方式降低，即通过超声和在不同温度下进行酸性水解，获得了MW在130 kDa至1300 kDa之间的样品。通过H-NMR测定DA，通过凝胶渗透色谱法测定MW。
