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用于简便制备酵母基免疫吸附剂的ZZ结构域的组成型细胞表面表达。

Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents.

作者信息

Katsurada Kohei, Tominaga Masahiro, Kaishima Misato, Kato Hiroko, Matsuno Toshihide, Ogino Chiaki, Kondo Akihiko, Ishii Jun, Takayama Katsumi

机构信息

Graduate School of Science, Technology and Innovation, Kobe University.

Department of Chemistry and Biology, National Institute of Technology (KOSEN), Fukui College.

出版信息

J Gen Appl Microbiol. 2021 Dec 31;67(6):265-268. doi: 10.2323/jgam.2021.03.004. Epub 2021 Aug 7.

DOI:10.2323/jgam.2021.03.004
PMID:34373371
Abstract

We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

摘要

我们描述了一种新型表达盒，它能够使源自金黄色葡萄球菌蛋白A的ZZ结构域在酵母细胞表面高效且组成型表达，从而轻松制备基于酵母的免疫吸附剂。利用这个包含PGK1启动子、源自α因子的分泌信号以及源自Flo1的锚定蛋白的表达盒，我们成功创建了一种用于人血清白蛋白的基于酵母的免疫吸附剂。

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J Gen Appl Microbiol. 2021 Dec 31;67(6):265-268. doi: 10.2323/jgam.2021.03.004. Epub 2021 Aug 7.
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引用本文的文献

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Development of a mammalian cell-based ZZ display system for IgG quantification.开发基于哺乳动物细胞的 ZZ 展示系统以定量 IgG。
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Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications.酵母表面展示系统：改进策略与生物技术应用
Front Bioeng Biotechnol. 2022 Jan 10;9:794742. doi: 10.3389/fbioe.2021.794742. eCollection 2021.