Department of Medicine, Division of Nephrology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Department of Orthopaedic Surgery, Indiana University Health Methodist Hospital, Indianapolis, IN 46202, USA.
Biotechniques. 2021 Aug;71(2):431-438. doi: 10.2144/btn-2021-0002. Epub 2021 Aug 10.
The ability to study the bone microenvironment of failed fracture healing may lead to biomarkers for fracture nonunion. Herein the authors describe a technique for isolating individual cells suitable for single-cell RNA sequencing analyses from intramedullary canal tissue collected by reaming during surgery. The purpose was to detail challenges and solutions inherent to the collection and processing of intramedullary canal tissue samples. The authors then examined single-cell RNA sequencing data from fresh and reanimated samples to demonstrate the feasibility of this approach for prospective studies.
研究骨折愈合失败的骨微环境的能力可能会导致骨折不愈合的生物标志物。在此，作者描述了一种从扩髓手术中收集的髓腔内组织中分离适合单细胞 RNA 测序分析的单个细胞的技术。目的是详细说明收集和处理髓腔内组织样本所固有的挑战和解决方案。然后，作者检查了新鲜和再激活样本的单细胞 RNA 测序数据，以证明这种方法用于前瞻性研究的可行性。
